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Related Experiment Video

Updated: May 24, 2026

High-plex Imaging using Spectral Confocal Microscopy to Minimize Non-specific Tissue Fluorescence

High-plex Imaging using Spectral Confocal Microscopy to Minimize Non-specific Tissue Fluorescence

Published on: October 28, 2025

Phase imaging flow cytometry using a focus-stack collecting microscope.

Sai Siva Gorthi¹, Ethan Schonbrun

¹Rowland Institute at Harvard, Harvard University, Cambridge, Massachusetts 02142, USA. gorthi@rowland.harvard.edu

|February 21, 2012

Summary

This summary is machine-generated.

A new microscope uses fluidics to efficiently collect focus stacks of single cells. This method enables quantitative phase imaging and measurement of 3D shape variations in red blood and leukemia cells.

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Area of Science:

Biophysics
Cell Biology
Microscopy

Background:

Quantitative phase imaging offers label-free cell analysis.
Collecting focus stacks of numerous cells efficiently is challenging.

Purpose of the Study:

To introduce a fluidics-based system for efficient cell focus stack collection.
To enable quantitative phase reconstruction and 3D shape analysis of cells.

Main Methods:

Development of a microfluidic device for cell transport through a microscope's focal plane.
Acquisition of focus stacks for individual cells.
Application of the transport-of-intensity-equation (TIE) for quantitative phase reconstruction.
Utilizing a phase imaging flow cytometer for cell analysis.

Main Results:

Demonstrated efficient collection of focus stacks for large cell populations.
Successfully reconstructed quantitative phase images of cells.
Measured three-dimensional shape variations in red blood cells and leukemia cells.

Conclusions:

The fluidics-based focus-stack collecting microscope provides an efficient platform for high-throughput cell imaging.
Quantitative phase imaging combined with this method allows for detailed analysis of cell morphology and dynamics.