Related Experiment Video
Updated: May 24, 2026
11:58
Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA
Published on: June 25, 2014
Constructing a DNA ladder Range for Lambda Phage by multiplex PCR
R Gopalakrishnan1, S Joseph, S Sellappa
1Department of Biotechnology, School of Life Sciences, Karpagam University, Coimbatore, Tamil Nadu, India.
Iranian Journal of Microbiology
|February 21, 2012
Summary
Researchers developed a cost-effective method to create DNA ladders using multiplex PCR and lambda phage DNA. This technique amplifies specific DNA fragments, providing a valuable alternative to commercial DNA molecular weight markers.
Area of Science:
- Molecular Biology
- Biotechnology
Background:
- DNA ladders are essential tools for determining the size of unknown DNA fragments.
- Various DNA ladders exist, tailored for specific DNA length ranges.
- Primer design targeting conserved sequences enables the generation of DNA fragments with known sizes.
Purpose of the Study:
- To describe a novel method for preparing DNA ladders.
- To utilize multiplex PCR for amplifying DNA fragments of specific sizes.
- To offer a cost-effective alternative to commercial DNA molecular weight markers.
Main Methods:
- A DNA ladder was prepared using multiplex polymerase chain reaction (PCR).
- Primers were designed based on the 1216-2136 sequence of lambda phage DNA.
- Specific DNA fragments were amplified and extracted.
Main Results:
- Multiplex PCR successfully amplified lambda phage DNA fragments.
- One forward and six different reverse primers were used for amplification.
- Successful amplification was achieved for fragments of 100, 200, 400, 600, 800, and 1000 base pairs (bp).
Conclusions:
- The described method provides a cost-effective approach to DNA ladder preparation.
- This technique offers a viable alternative to expensive commercial DNA molecular weight markers.
