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Mating type and genotyping of Candida albicans isolated from different samples
Abed Karimpour1, Maral Gharaghani2, Amir Kamal Hardani3
1Department of Medical Mycology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Background And Objectives:
Candida albicans is a common opportunistic pathogen. Genotyping based on the 25S rDNA and mating type locus (MTL) allows for epidemiological and genetic profiling. This study aimed to characterize the genotypes and mating types of C. albicans isolates from various clinical sources in Iran.
Materials And Methods:
Ninety-four isolates from clinical samples (saliva, urine, vaginal swabs, and skin scrapings) were cultured on CHROMagar Candida and identified by standard phenotypic methods. Genotyping was performed using CAINT primers, and mating type analysis was conducted using MTLa1 and MTLα1 primers.
Results:
In this study, 94 isolates of C. albicans from various sources were analyzed. Genotype A was the most frequent (65%), followed by genotypes C (24.5%), B (9.6%), and D (1.1%). Most isolates (97.9%) were heterozygous at the MTL locus, only two isolates homozygous (α/α).
Conclusion:
Genotype A and MTL-heterozygous strains were predominant among C. albicans isolates, suggesting a consistent molecular pattern across different clinical sources and regions.
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