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Flow cytometric analysis of cells using an antibody to a surface antigen

M G Ormerod1

  • 1Institute of Cancer Research, The Haddow Laboratories, Sutton, Surrey, UK.

Methods in Molecular Biology (Clifton, N.J.)
|February 22, 2012
PubMed

Identifying a subset of cells, particularly peripheral blood lymphocytes, by means of a fluorescently-tagged antibody is one of the commonest applications of flow cytometry. There are many methods, all of them basically similar, for labeling antigens on cell surfaces. Two slightly different procedures are given in this chapter; these can be adapted to meet most needs.

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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