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Related Concept Videos

Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...
Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Related Experiment Video

Updated: May 16, 2026

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland
11:13

Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland

Published on: December 1, 2015

Enzyme-antienzyme method for immunohistochemistry.

M G Ormerod1, S F Imrie

  • 1The Haddow Laboratories, Institute of Cancer Research, Sutton, Surrey, UK.

Methods in Molecular Biology (Clifton, N.J.)
|November 15, 2012
PubMed
Summary
This summary is machine-generated.

Immunohistochemical stains utilize enzyme-labeled antibodies for precise tissue analysis. This method allows for nuclear counterstaining to visualize tissue architecture and provides long-lasting, stable staining for archival purposes.

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Last Updated: May 16, 2026

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Published on: December 1, 2015

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections
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Published on: November 14, 2016

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Area of Science:

  • Biomedical Sciences
  • Histology
  • Immunohistochemistry

Background:

  • Immunohistochemical stains are crucial for identifying specific cellular and tissue components.
  • Antibodies are key reagents, requiring a detection system to visualize their binding sites.

Purpose of the Study:

  • To explain the principles of immunohistochemical staining.
  • To highlight the advantages of enzyme-labeled antibodies over fluorescent labels.

Main Methods:

  • Utilizing antibodies to target specific constituents within tissue sections.
  • Employing enzyme-labeled antibodies reacted with substrates to generate colored products for visualization.
  • Considering fluorescent labels as an alternative detection method.

Main Results:

  • Enzyme labels enable nuclear counterstaining, which reveals overall tissue architecture.
  • Enzyme-labeled stains exhibit slow fading, ensuring long-term slide stability and storage.

Conclusions:

  • Enzyme-labeled immunohistochemistry offers superior visualization of tissue architecture through counterstaining.
  • The stability of enzyme-based stains facilitates slide archiving and future analysis.