Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Probe and Assay Dependent Pharmacology of Dantrolene Analogues at the GPR35.

ACS medicinal chemistry letters·2026
Same author

Multimodal opioid-free anesthesia containing esketamine versus opioid-based anesthesia: a systematic review and meta-analysis.

BMC anesthesiology·2026
Same author

Efficacy, tolerability, and safety of 1.5L-lactulose as bowel preparation for patients with inflammatory bowel disease: a randomized, single-blinded trial.

Internal and emergency medicine·2026
Same author

Application of the PDCA cycle management in quality improvement of endotracheal intubation in emergency department among standardised training residents.

BMC medical education·2026
Same author

Ellagic acid alleviates ulcerative colitis in a GPR35-dependent manner associated with SDH restoration.

Phytomedicine : international journal of phytotherapy and phytopharmacology·2026
Same author

N-Glycosylation and Alzheimer's disease: A 2001-2025 global bibliometric landscape revealing emerging diagnostic trends.

Journal of Alzheimer's disease reports·2026

Related Experiment Video

Updated: May 24, 2026

Stepwise Dosing Protocol for Increased Throughput in Label-Free Impedance-Based GPCR Assays
06:13

Stepwise Dosing Protocol for Increased Throughput in Label-Free Impedance-Based GPCR Assays

Published on: February 21, 2020

Modulating cell-cell communication with a high-throughput label-free cell assay.

Guangshan Li1, Fang Lai, Ye Fang

  • 1Corning Inc., Biochemical Technologies, Corning, NY 14831, USA. lig2@corning.com

Journal of Laboratory Automation
|February 24, 2012
PubMed
Summary

This study introduces a label-free cell assay using the Epic® system to monitor cell-cell communication. The assay effectively measures natural killer (NK) cell cytotoxicity against cancer cells, aiding in the discovery of new therapeutic compounds.

More Related Videos

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
09:09

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors
10:59

Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors

Published on: February 10, 2014

Related Experiment Videos

Last Updated: May 24, 2026

Stepwise Dosing Protocol for Increased Throughput in Label-Free Impedance-Based GPCR Assays
06:13

Stepwise Dosing Protocol for Increased Throughput in Label-Free Impedance-Based GPCR Assays

Published on: February 21, 2020

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
09:09

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors
10:59

Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors

Published on: February 10, 2014

Area of Science:

  • Biotechnology
  • Cell Biology
  • Immunology

Background:

  • Natural killer (NK) cells are crucial for identifying and eliminating abnormal cells, including cancer cells.
  • Intercellular adhesion molecule 1 (ICAM1) plays a role in cell recognition and interaction.
  • Monitoring cell-cell communication is vital for understanding immune responses and disease progression.

Purpose of the Study:

  • To demonstrate a high-throughput, label-free cell assay for quantifying cell-cell communication.
  • To examine the cytotoxic effects of NK92MI effector cells on HeLa and CHO-ICAM1 target cells.
  • To establish the assay's utility in discovering novel chemicals that modulate cell-cell interactions.

Main Methods:

  • Utilized the Epic® resonant waveguide grating sensor platform for real-time monitoring.
  • Measured cell responses as wavelength shifts in picometers.
  • Investigated the impact of effector-to-target cell ratio (E/T), interaction time, and target cell type on assay detectability.

Main Results:

  • NK92MI cell-mediated cytotoxicity against target cells resulted in negative optical signals, linked to caspase-dependent apoptosis.
  • Different E/T ratios and target cell types (HeLa vs. CHO-ICAM1) influenced signal detectability.
  • Pretreatment of target cells with pharmaceutical reagents generated distinct optical signals, validating the assay's sensitivity.

Conclusions:

  • The developed label-free assay provides a sensitive method for real-time monitoring of cell-cell communication and cytotoxicity.
  • The assay is effective in quantifying NK cell activity and can be adapted for drug discovery targeting cell-cell interactions.
  • This platform facilitates the identification of compounds that modulate immune cell responses and cancer cell apoptosis.