Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Introduction to Nuclear Reprogramming01:14

Introduction to Nuclear Reprogramming

Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ASO Visual Abstract: Immediate Second Look After Cytoreduction for Colorectal or Ovarian Carcinomatosis: An Invisible Gorilla Effect?

Annals of surgical oncology·2026
Same author

Immediate Second Look After Cytoreduction for Colorectal or Ovarian Carcinomatosis: An Invisible Gorilla Effect?

Annals of surgical oncology·2026
Same author

PIPAC's Path: When Details Drive Progress.

Annals of surgical oncology·2026
Same author

Corrigendum to 'Osteoporosis prediction from frontal lumbar spine X-rays' [Journal of clinical densitometry, volume 29 (2026), 101666].

Journal of clinical densitometry : the official journal of the International Society for Clinical Densitometry·2026
Same author

Deciphering the Nanoscale Architecture of Presynaptic Actin Using a Micropatterned Presynapse-on-Glass Model.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2026
Same author

Osteoporosis prediction from Frontal Lumbar Spine X-rays.

Journal of clinical densitometry : the official journal of the International Society for Clinical Densitometry·2026
Same journal

Disentangling the response to lysosomal damage.

Journal of cell science·2026
Same journal

The force, form and function of the nucleus.

Journal of cell science·2026
Same journal

The nucleus-vacuole junction at a glance.

Journal of cell science·2026
Same journal

Loss of INPP5E affects photoreceptor outer segment membrane biogenesis in iPSC-derived human retinal organoids.

Journal of cell science·2026
Same journal

Brinker regulates reciprocal outcomes of BMP signal between stem cells and differentiating cells.

Journal of cell science·2026
Same journal

Primary cilium disassembly - from mechanisms to roles in physiology and disease.

Journal of cell science·2026
See all related articles

Related Experiment Video

Updated: May 24, 2026

Control of Cell Geometry through Infrared Laser Assisted Micropatterning
11:04

Control of Cell Geometry through Infrared Laser Assisted Micropatterning

Published on: July 10, 2021

Reprogramming cell shape with laser nano-patterning.

Timothée Vignaud1, Rémi Galland, Qingzong Tseng

  • 1Laboratoire de Physiologie Cellulaire et Végétale, Institut de Recherche en Technologies et Sciences pour le Vivant, CNRS/UJF/INRA/CEA, Grenoble, France.

Journal of Cell Science
|February 24, 2012
PubMed
Summary
This summary is machine-generated.

Researchers used a pulsed laser to dynamically control cell shape by creating new adhesion sites in real time. This method precisely manipulates cell architecture and actin structures, offering new insights into cell behavior.

More Related Videos

Simple Lithography-Free Single Cell Micropatterning using Laser-Cut Stencils
08:59

Simple Lithography-Free Single Cell Micropatterning using Laser-Cut Stencils

Published on: April 3, 2020

Expanding Nanopatterned Substrates Using Stitch Technique for Nanotopographical Modulation of Cell Behavior
09:06

Expanding Nanopatterned Substrates Using Stitch Technique for Nanotopographical Modulation of Cell Behavior

Published on: December 8, 2016

Related Experiment Videos

Last Updated: May 24, 2026

Control of Cell Geometry through Infrared Laser Assisted Micropatterning
11:04

Control of Cell Geometry through Infrared Laser Assisted Micropatterning

Published on: July 10, 2021

Simple Lithography-Free Single Cell Micropatterning using Laser-Cut Stencils
08:59

Simple Lithography-Free Single Cell Micropatterning using Laser-Cut Stencils

Published on: April 3, 2020

Expanding Nanopatterned Substrates Using Stitch Technique for Nanotopographical Modulation of Cell Behavior
09:06

Expanding Nanopatterned Substrates Using Stitch Technique for Nanotopographical Modulation of Cell Behavior

Published on: December 8, 2016

Area of Science:

  • Cell biology
  • Biophysics
  • Materials science

Background:

  • Cell shape is crucial for cellular functions and is influenced by the extracellular matrix.
  • Existing micropatterning techniques offer fixed designs, failing to mimic the dynamic nature of the cellular microenvironment.
  • Understanding cell shape regulation is key to fields like tissue engineering and developmental biology.

Purpose of the Study:

  • To develop a real-time method for dynamically controlling living cell shape.
  • To investigate the precise geometric cues that regulate cell shape extension and contraction.
  • To precisely control and study the organization of actin-based cellular structures.

Main Methods:

  • Utilized a tightly focused pulsed laser to create dynamic, geometrically defined adhesion sites on cell culture substrates.
  • Achieved sub-micrometer resolution patterning to precisely control the placement of adhesion sites.
  • Observed and manipulated cellular responses, including actin structure formation and reorganization, in real time.

Main Results:

  • Demonstrated real-time manipulation of cell shape by introducing dynamic adhesion sites.
  • Identified critical distances between adhesion sites influencing cell shape extension and contraction.
  • Successfully induced, displaced, and removed specific actin-based structures like filament bundles and branched meshworks.
  • Showed that isotropic actin meshworks could be directed to form polarized stress fibers in response to geometric cues.

Conclusions:

  • The laser-based dynamic micropatterning method provides precise, real-time control over cell shape and architecture.
  • This technique allows for the investigation of fundamental cell mechanics and the role of geometric cues in regulating cellular structures.
  • Offers a versatile tool for studying cell behavior and developing advanced biomaterials.