Hyphenated techniques and their applications in natural products analysis

Area of Science:

• Analytical Chemistry
• Natural Product Chemistry
• Spectroscopy

Background:

• Hyphenated techniques couple separation methods with online spectroscopic detection (e.g., GC-MS, LC-MS).
• Recent advancements have expanded their use in analyzing complex biomaterials, especially natural products.
• Liquid chromatography (LC) based hyphenated techniques are particularly emphasized.

Purpose of the Study:

• To review the applications of hyphenated techniques in natural product research.
• To highlight their role in pre-isolation, isolation, and dereplication of compounds.
• To showcase their utility in chemotaxonomic studies, chemical fingerprinting, quality control, and metabolomics.

Main Methods:

• Utilizing hyphenated analytical techniques, with a focus on LC-based methods.
• Coupling separation techniques (e.g., LC, GC, CE) with spectroscopic detectors (e.g., MS, PDA, FTIR, NMR).
• Applying these techniques for various analytical tasks including isolation and identification.

Main Results:

• Demonstrated broad applicability of hyphenated techniques across multiple natural product analysis areas.
• Provided specific examples of successful applications in the literature.
• Highlighted the effectiveness of LC-coupled hyphenated methods for complex samples.

Conclusions:

• Hyphenated techniques are powerful tools for the comprehensive analysis of natural products.
• These methods significantly advance research in isolation, identification, and quality assessment.
• The integration of separation and detection offers unparalleled insights into complex natural product mixtures.