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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Related Experiment Video

Updated: May 24, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Lipolytic proteomics.

Matthias Schittmayer1, Ruth Birner-Gruenberger

  • 1Research Group Functional Proteomics, Institute of Pathology, Medical University of Graz, Graz, Austria.

Mass Spectrometry Reviews
|March 7, 2012
PubMed
Summary
This summary is machine-generated.

Activity-based proteomics (ABP) uses molecular probes to study lipases, enzymes crucial for lipolysis. This method simplifies complex samples, enabling detailed analysis of lipase activity and identification of key proteins.

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Area of Science:

  • Biochemistry
  • Proteomics
  • Enzymology

Background:

  • Activity-based proteomics (ABP) utilizes molecular probes to target enzymes by their catalytic mechanisms.
  • Most lipases are serine hydrolases with a conserved active-site serine, making them suitable for ABP.
  • ABP significantly reduces sample complexity by isolating specific enzyme subproteomes.

Purpose of the Study:

  • To review the design, technological advancements, and applications of ABP for lipase research.
  • To provide an overview of proteins identified using ABP in lipase studies.
  • To highlight the utility of ABP in studying lipolysis.

Main Methods:

  • Review of literature on activity-based probes for lipases.
  • Discussion of technological developments in ABP.
  • Analysis of applications and identified proteins in lipase-focused ABP studies.

Main Results:

  • ABP probes are effective for studying lipases due to their shared catalytic serine.
  • ABP enables in-depth proteomic analysis by reducing sample complexity.
  • A comprehensive overview of probe design, technology, and applications is presented.

Conclusions:

  • Activity-based proteomics is a powerful tool for investigating lipases and lipolysis.
  • ABP facilitates the identification and characterization of lipases within complex biological systems.
  • Technological advancements continue to enhance the application of ABP in enzyme research.