Related Experiment Video
Updated: May 24, 2026
07:50
Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina
Published on: August 29, 2018
Histone demethylase JMJD5 is essential for embryonic development.
1Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
Biochemical and Biophysical Research Communications
|March 10, 2012
Summary
Histone demethylase JMJD5 (jumonji C domain-containing 5) is crucial for embryonic development. Its absence leads to p53 activation and embryonic lethality, suggesting JMJD5
Area of Science:
- Epigenetics and Developmental Biology
- Molecular Oncology
Background:
- Histone lysine methylation regulates chromatin structure and gene expression.
- JMJD5 (jumonji C domain-containing 5) is a histone demethylase that removes methyl groups from histone H3 lysine 36.
- JMJD5 promotes proliferation in breast cancer cells.
Purpose of the Study:
- To investigate the physiological role of JMJD5 using knockout mouse models.
- To understand the impact of JMJD5 deficiency on embryonic development and gene expression.
Main Methods:
- Generation of JMJD5 knockout mice (heterozygous and homozygous).
- Phenotypic analysis of developing embryos at various stages (E8.5, E10.5).
- Quantitative analysis of gene expression, including p53, p21, and Noxa.
Main Results:
- Homozygous JMJD5 knockout mice exhibit embryonic lethality around day 10 of development.
- JMJD5(-/-) embryos show delayed development and are actively resorbed.
- Lack of JMJD5 leads to upregulation of the tumor suppressor p53 and its target genes (p21, Noxa).
Conclusions:
- JMJD5 is essential for normal embryonic development.
- JMJD5 acts as a repressor of p53 expression.
- JMJD5's role in repressing p53 suggests oncogenic activity, consistent with its upregulation in cancers.
Related Concept Videos
01:10Zygotic Development And Stem Cell Formation
The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...
00:58Embryonic Stem Cells
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
00:57Embryonic Stem Cells
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
02:23Master Transcription Regulators
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
02:23Master Transcription Regulators
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
02:32Histone Modification
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...