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Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers
Published on: September 19, 2022
CPPsite: a curated database of cell penetrating peptides.
Ankur Gautam1, Harinder Singh, Atul Tyagi
1Bioinformatics Centre, Institute of Microbial Technology (CSIR), Sector 39-A, Chandigarh, India.
Database : the Journal of Biological Databases and Curation
|March 10, 2012
Summary
Cell penetrating peptides (CPPs) facilitate drug delivery into cells. A new database, CPPsite, compiles 843 experimentally validated CPPs, aiding future drug development research.
Area of Science:
- Biochemistry
- Molecular Biology
- Drug Delivery
Background:
- Cellular drug delivery remains a significant hurdle in pharmaceutical development.
- Cell penetrating peptides (CPPs) offer a promising strategy for intracellular delivery of therapeutic molecules across diverse cell types.
- The unique ability of CPPs to penetrate cell membranes highlights their therapeutic potential.
Purpose of the Study:
- To establish CPPsite, a comprehensive, manually curated database of experimentally validated cell penetrating peptides.
- To consolidate information from scientific literature and patents regarding CPPs.
- To provide a centralized resource for researchers in the field of drug delivery.
Main Methods:
- Manual curation of 843 experimentally validated CPPs from literature and patents.
- Inclusion of detailed peptide information: ID, PubMed ID, sequence, chirality, origin, nature, sub-cellular localization, uptake efficiency, mechanism, hydrophobicity, and amino acid composition.
- Development of user-friendly tools for searching, browsing, analyzing, and mapping CPP data.
Main Results:
- Creation of CPPsite, a manually curated database containing 843 experimentally validated CPPs.
- Detailed annotation for each peptide, encompassing sequence, properties, and functional characteristics.
- Integration of analytical tools for sequence and property analysis, including secondary/tertiary structure prediction and physicochemical property determination.
Conclusions:
- CPPsite serves as a valuable resource for the scientific community, consolidating critical data on cell penetrating peptides.
- The database facilitates the exploration of CPP characteristics and aids in the development of predictive models for novel CPPs.
- CPPsite is expected to accelerate research in drug delivery and the development of new therapeutic strategies.
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