Related Experiment Video
Updated: May 24, 2026

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
Published on: July 28, 2017
Plastid DNA in the nucleus: new genes for old
Mathieu Rousseau-Gueutin1, Michael A Ayliffe, Jeremy N Timmis
1School of Molecular and Biomedical Science, The University of Adelaide, Adelaide, SA, Australia. mathieu.rousseau@adelaide.edu.au
Abstract:
Nuclear genomes of eukaryotes are bombarded by a continuous deluge of organellar DNA which contributes significantly to eukaryote evolution. Here, we present a new PCR-based method that allows the specific amplification of nuclear integrants of organellar DNA (norgs) by exploiting recent deletions present in organellar genome sequences. We have used this method to amplify nuclear integrants of plastid DNA (nupts) from the nuclear genomes of several Nicotiana species and to study the evolutionary forces acting upon these sequences. The role of nupts in endosymbiotic evolution and the different genetic factors influencing the time available for a chloroplastic gene to be functionally relocated in the nucleus are discussed.
Related Concept Videos
Non-nuclear Inheritance
Non-nuclear Inheritance
Export of Mitochondrial and Chloroplast Genes
Genome Size and the Evolution of New Genes
Genome Size and the Evolution of New Genes
Methods of Nuclear Reprogramming

