Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 24, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Determination of single cell surface protein expression using a tagless microfluidic method.

Rajan Kumar1, Sri HariKrishna Vellanki, Robin Smith

  • 1Genome Data Systems, Inc., Hamilton, NJ, USA.

Lab on a Chip
|March 15, 2012
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Background-free <sup>12</sup>C(<i>α</i>, <i>γ</i>) angular distribution measurements with a time projection chamber operating in Gamma beams.

Communications physics·2026
Same author

Comparative analysis of phytochemicals in different plant organs of <i>Verbascum thapsus</i> L. by using UPLC/MS-QToF and analytical standardization of bioactive compounds, verbascoside and luteolin, on HPLC platform.

Natural product research·2026
Same author

Clinical spectrum and associated comorbidities of cerebral palsy among children in Eastern India.

Scientific reports·2025
Same author

Asthma beyond the clinic: a decade-long narrative review of parental knowledge and practices in Indian children.

Monaldi archives for chest disease = Archivio Monaldi per le malattie del torace·2025
Same author

Carbazole-imidazole synergy for discriminative BSA sensing and a self-assembled organogel.

Journal of materials chemistry. B·2025
Same author

The Impact of AVATAR Therapy on Voice Hearing in Everyday Life: ESM Outcomes of the AVATAR2 Trial.

Schizophrenia bulletin·2025
Same journal

Tunable self-assembling cellular microarray for single-neutrophil vital and suicidal extracellular traps.

Lab on a chip·2026
Same journal

Precise programmable tumor cell subpopulation sorting <i>via</i> an electromagnetic microfluidic platform.

Lab on a chip·2026
Same journal

Bridging dimensions: combining one- and two-photon 3D printing for microfluidic device fabrication.

Lab on a chip·2026
Same journal

Microfluidic rare cell analysis beyond counting: workflow design from enrichment to multi-omics.

Lab on a chip·2026
Same journal

A sperm racetrack to separate sperm by swim speed.

Lab on a chip·2026
Same journal

Controlled encapsulation and droplet size prediction in two-step microfluidic double emulsions.

Lab on a chip·2026
See all related articles

This study introduces a novel microfluidic method to detect specific surface proteins on single cells without labeling. The technique accurately identifies cancer cells expressing interleukin 13 receptor alpha 2 (IL13Rα2).

Area of Science:

  • Biotechnology
  • Cell Biology
  • Microfluidics

Background:

  • Detecting surface protein expression is crucial for cell analysis, particularly in cancer diagnostics.
  • Current methods often require cell labeling or manipulation, which can lead to cell loss and altered characteristics.
  • There is a need for non-destructive, high-sensitivity methods for single-cell surface protein detection.

Purpose of the Study:

  • To develop and validate a label-free microfluidic method for detecting surface protein expression in single cells.
  • To assess the sensitivity and specificity of this method for identifying specific cancer cell markers.
  • To demonstrate the potential for multiplexed analysis and non-destructive cell recovery.

Main Methods:

  • Utilizing a microfluidic device where cells flow past immobilized ligands specific to surface proteins.

More Related Videos

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
09:09

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Counting Proteins in Single Cells with Addressable Droplet Microarrays
12:25

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Published on: July 6, 2018

Related Experiment Videos

Last Updated: May 24, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
09:09

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Counting Proteins in Single Cells with Addressable Droplet Microarrays
12:25

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Published on: July 6, 2018

  • Measuring the transient binding events by detecting a decrease in individual cell velocity.
  • Applying the method to detect interleukin 13 receptor alpha 2 (IL13Rα2) on a metastatic breast cancer cell line (LM2).

    • Main Results:

    • The method achieved near 100% sensitivity and 100% specificity in detecting IL13Rα2 expression on LM2 cells.
    • Proof of principle for multiplexing was demonstrated by tracking cells over differentially coated microfluidic patches.
    • The non-destructive nature of the assay allows for cell collection and reanalysis.

    Conclusions:

    • This label-free microfluidic approach enables accurate and efficient detection of surface protein expression in single cells.
    • The technology shows significant potential for analyzing rare cells, such as in needle aspirates, and for multiplexed biomarker detection.
    • The non-destructive nature preserves cell integrity for further downstream applications.