Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An achromatic neutron lens.

Nature communications·2026
Same author

Paired CycleGAN-based virtual staining for 3D X-ray histology of bone-implant systems.

Journal of synchrotron radiation·2026
Same author

An induction heating system for in situ X-ray diffraction imaging: design, simulation and application to dislocation dynamics in semiconductors.

Journal of synchrotron radiation·2026
Same author

Extending the Field of View in Modulation-Based X-Ray Phase Microtomography.

IEEE transactions on image processing : a publication of the IEEE Signal Processing Society·2026
Same author

High-throughput phenomics of global ant biodiversity.

Nature methods·2026
Same author

Quantitative Stain Mapping in X-Ray Virtual Histology.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Related Experiment Video

Updated: May 24, 2026

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

Phase contrast laminography based on Talbot interferometry.

Venera Altapova1, Lukas Helfen, Anton Myagotin

  • 1Laboratorium f¨ur Applikationen der Synchrotronstrahlung (LAS), Faculty of Physics, Karlsruher Institut f¨ur Technologie (KIT), Postfach 6980, D-76128 Karlsruhe, Germany. venera.altapova@kit.edu

Optics Express
|March 16, 2012
PubMed
Summary
This summary is machine-generated.

This study introduces a novel X-ray technique combining synchrotron laminography and Talbot grating interferometry for analyzing weakly absorbing materials. The method enables non-destructive 3-D imaging, revealing absorption, phase, and dark-field contrasts for diverse applications, including cultural heritage.

More Related Videos

Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating
10:39

Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating

Published on: October 11, 2016

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)
11:57

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)

Published on: December 1, 2016

Related Experiment Videos

Last Updated: May 24, 2026

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating
10:39

Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating

Published on: October 11, 2016

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)
11:57

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)

Published on: December 1, 2016

Area of Science:

  • Physics
  • Materials Science
  • Imaging Technology

Background:

  • Analyzing weakly absorbing specimens with X-rays presents challenges for traditional imaging methods.
  • Macroscopically large, flat samples require advanced techniques for detailed inspection.
  • Non-destructive 3-D imaging is crucial for preserving delicate materials, especially in cultural heritage.

Purpose of the Study:

  • To develop and implement a novel X-ray diagnostic technique by integrating synchrotron laminography with Talbot grating interferometry.
  • To enable multiple contrast screening and non-destructive 3-D inspection of large, weakly absorbing specimens.
  • To demonstrate the technique's potential in cultural heritage research, specifically for historical parchment analysis.

Main Methods:

  • Combination of synchrotron laminography and Talbot grating interferometry in a single setup.
  • Simultaneous acquisition of absorption, phase, and dark-field contrast data.
  • Reconstruction of 3-D contrast maps for detailed analysis.

Main Results:

  • Successful integration of synchrotron laminography and Talbot grating interferometry.
  • Generation of simultaneous 3-D absorption, phase, and dark-field contrast reconstructions.
  • Demonstration of the technique's capability for analyzing weakly absorbing materials.

Conclusions:

  • The integrated X-ray technique offers a powerful tool for multi-contrast screening and 3-D inspection of large, weakly absorbing samples.
  • This novel method has significant potential for applications in materials science and cultural heritage preservation.
  • The study provides a foundation for further development and application of this advanced X-ray imaging modality.