Liquid chromatography-flame ionisation detection using a nebuliser/spray chamber interface. Part 2. Comparison of
Erepamowei Young1, Roger M Smith, Barry L Sharp
1Department of Chemistry, Loughborough University, Loughborough, Leicestershire LE11 3TU, UK.
Journal of Chromatography. A
|March 17, 2012
Summary
Superheated water liquid chromatography coupled with flame ionization detection shows promise for analyzing various compounds. While effective for volatile analytes, it struggles with non-volatile substances like amino acids.
Area of Science:
- Analytical Chemistry
- Chromatography
- Spectroscopy
Background:
- Superheated water liquid chromatography (SHWLC) offers an environmentally friendly alternative to organic solvents.
- Flame ionization detection (FID) is a sensitive detection method commonly used in gas chromatography (GC-FID).
- Integrating LC with FID requires specialized interfaces to handle liquid effluents.
Purpose of the Study:
- To evaluate the performance of a nebulizer/spray chamber interface for flame ionization detection (FID) in superheated water liquid chromatography (SHWLC).
- To compare the linearity and sensitivity of FID response for volatile and involatile analytes in SHWLC.
- To assess the suitability of SHWLC-FID for analyzing a diverse range of aliphatic and aromatic compounds.
Main Methods:
- Utilized a liquid chromatography (LC) system with superheated water as the mobile phase.
- Employed a nebulizer/spray chamber interface to couple the LC system with a flame ionization detector (FID).
- Analyzed various aliphatic and aromatic compounds, including volatile and involatile analytes like amino acids and carbohydrates.
Main Results:
- Demonstrated the applicability of SHWLC-FID for a broad spectrum of aliphatic and aromatic analytes.
- Observed similar detector responses to different analytes compared to GC-FID.
- Found FID response for volatile analytes to be comparable to UV detection.
- Reported poor and often lower responses for involatile analytes (e.g., amino acids, carbohydrates) compared to a refractive index detector.
Conclusions:
- SHWLC-FID is a viable technique for analyzing volatile compounds, offering comparable sensitivity to UV detection.
- The method exhibits limitations for involatile analytes, with FID performance being inferior to refractive index detection.
- Further interface or detector development may be needed to improve SHWLC-FID performance for non-volatile substances.
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