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SDS-PAGE

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...

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Updated: Feb 2, 2026

Standardized SDS-PAGE Workflow for Personalized Protein Corona Profiling in Early Cancer Detection

Standardized SDS-PAGE Workflow for Personalized Protein Corona Profiling in Early Cancer Detection

Published on: December 19, 2025

Tricine-SDS-PAGE.

Syed R Haider¹, Helen J Reid¹, Barry L Sharp²

¹Department of Chemistry, Centre for Analytical Science, Loughborough University, Loughborough, UK.

Methods in Molecular Biology (Clifton, N.J.)

|November 15, 2018

Summary

This summary is machine-generated.

We developed a simplified tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (tricine-SDS-PAGE) method for separating low molecular mass proteins. This improved technique enhances compatibility with detectors like ICP-MS for easier quantitative analysis.

Keywords:

Gel buffer Gel mixture Low molecular mass proteins Low percentage gel Polyacrylamide Protein standard Running buffer Tricine-SDS-PAGE

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Area of Science:

Biochemistry
Analytical Chemistry
Proteomics

Background:

Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (tricine-SDS-PAGE) is established for separating low molecular mass proteins.
The standard tricine-SDS-PAGE protocol can be complex and hinder protein recovery for subsequent analysis.

Purpose of the Study:

To present a simplified tricine-SDS-PAGE system for efficient separation of small proteins.
To improve the compatibility of the separation method with common analytical detectors.

Main Methods:

Development of a simplified tricine-SDS-PAGE protocol.
Utilizing lower percentage polyacrylamide gels.
Assessing compatibility with UV and inductively coupled plasma-mass spectrometry (ICP-MS).

Main Results:

The simplified tricine-SDS-PAGE effectively separates low molecular mass proteins.
The modified system demonstrates high compatibility with UV and ICP-MS detection.
Facilitates easier recovery of proteins from gels for quantitative analysis.

Conclusions:

The simplified tricine-SDS-PAGE offers an efficient and accessible method for small protein separation.
This technique is suitable for quantitative proteomic studies requiring protein recovery and sensitive detection.