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Tricine-SDS-PAGE

Syed R Haider¹, Helen J Reid¹, Barry L Sharp²

¹Department of Chemistry, Centre for Analytical Science, Loughborough University, Loughborough, UK.

Methods in Molecular Biology (Clifton, N.J.)

|November 15, 2018

Abstract:

Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (tricine-SDS-PAGE) is an efficient way of separating low molecular mass proteins. However, the standard system is quite complicated and specifically may not be useful when the separated proteins are to be recovered from the gel for quantitative analysis. Here, we describe a simplified system whereby these smaller proteins can be resolved in comparatively low percentage gels which have high compatibility with modern detectors such as UV and inductively coupled plasma-mass spectrometry (ICP-MS).

Keywords:

Gel buffer Gel mixture Low molecular mass proteins Low percentage gel Polyacrylamide Protein standard Running buffer Tricine-SDS-PAGE

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SDS-PAGE

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...

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