Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
NF-kB-dependent Signaling Pathway02:26

NF-kB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pregnancy-induced tissue-resident memory-like T cells contribute to tumor control in breast cancer.

Nature immunology·2026
Same author

Mice lacking NF-ĸB1 undergo premature ovarian aging.

Reproductive biology and endocrinology : RB&E·2026
Same author

Therapeutic Silencing of Tmprss6 Reduces Iron-Induced Inflammation and Prolongs Survival in MDS Mice.

American journal of hematology·2026
Same author

The long non-coding RNA <i>Dreg1</i> is required for optimal ILC2 development.

eLife·2026
Same author

Three-dose COVID-19 vaccination elicits immune memory in patients with inborn errors of immunity even in the absence of neutralizing antibodies.

The Journal of allergy and clinical immunology·2026
Same author

The Various Shades of Hybridism in Rhinoplasty: Variations on Dorsal Preservation and Presentation of a Cartilaginous Pushdown Technique.

Facial plastic surgery : FPS·2026

Related Experiment Video

Updated: May 23, 2026

NF-&#954;B-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells
10:57

NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells

Published on: January 12, 2020

NF-κB subunit specificity in hemopoiesis.

Steve Gerondakis1, Ashish Banerjee, George Grigoriadis

  • 1Burnet Institute, Prahran, Vic., Australia. gerondakis@burnet.edu.au and

Immunological Reviews
|March 23, 2012
PubMed
Summary

Nuclear factor-kappa B (NF-κB) transcription factors are crucial for immune cell development. This review details NF-κB

More Related Videos

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/&#945;
11:27

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α

Published on: November 2, 2018

Methods for the Modulation and Analysis of NF-&#954;B-dependent Adult Neurogenesis
14:58

Methods for the Modulation and Analysis of NF-κB-dependent Adult Neurogenesis

Published on: February 13, 2014

Related Experiment Videos

Last Updated: May 23, 2026

NF-&#954;B-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells
10:57

NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells

Published on: January 12, 2020

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/&#945;
11:27

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α

Published on: November 2, 2018

Methods for the Modulation and Analysis of NF-&#954;B-dependent Adult Neurogenesis
14:58

Methods for the Modulation and Analysis of NF-κB-dependent Adult Neurogenesis

Published on: February 13, 2014

Area of Science:

  • Immunology
  • Cell Biology
  • Developmental Biology

Background:

  • The nuclear factor-kappa B (NF-κB) pathway is essential for cellular stress responses.
  • NF-κB transcription factors also play critical roles in the development of various immune cell lineages.

Purpose of the Study:

  • To review the multifaceted roles of NF-κB transcription factors in hemopoietic cell differentiation.
  • To highlight NF-κB's involvement in the development of T-cell and myeloid lineages.

Main Methods:

  • Literature review of studies on NF-κB function in hematopoiesis.
  • Analysis of NF-κB's regulatory mechanisms in immune cell differentiation.

Main Results:

  • NF-κB controls key aspects of thymic T-cell differentiation, including conventional αβ T cells, regulatory T cells, natural killer T cells, and γδ T cells.
  • NF-κB is vital for the development of myeloid cells such as macrophages and dendritic cells.

Conclusions:

  • NF-κB transcription factors are indispensable regulators of both T-cell and myeloid differentiation.
  • Understanding NF-κB's role provides insights into immune system development and potential therapeutic targets.