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Calcium sensing receptor activation elevates proinflammatory factor expression in human adipose cells and adipose
Mariana Cifuentes1, Cecilia Fuentes, Nicolas Tobar
1Institute of Nutrition and Food Technology (INTA), Universidad de Chile, Santiago, Chile. mcifuentes@inta.uchile.cl
Molecular and Cellular Endocrinology
|March 28, 2012
Summary
Obesity-related inflammation in fat tissue involves the calcium sensing receptor (CaSR). CaSR activation increases inflammatory factors, partly via nuclear factor kappa B (NFκB), suggesting therapeutic potential.
Area of Science:
- Biochemistry
- Cell Biology
- Endocrinology
Background:
- Adipose tissue inflammation is linked to obesity's metabolic and cardiovascular issues.
- The calcium sensing receptor (CaSR) is present in human fat cells and upregulated during obesity-related inflammation.
Purpose of the Study:
- To investigate the role of CaSR in promoting inflammatory factor expression in adipose tissue.
- To explore the potential of CaSR as a therapeutic target for obesity-induced adipose tissue dysfunction.
Main Methods:
- Activation of CaSR using cinacalcet in adipose tissue and LS14 cells.
- Measurement of inflammatory cytokine and chemokine expression (IL6, IL1β, TNFα, CCL2).
- Inhibition of nuclear factor kappa B (NFκB) pathway using SN50.
Main Results:
- CaSR activation significantly increased the expression of proinflammatory cytokines IL6, IL1β, TNFα, and chemokine CCL2.
- The observed increase in inflammatory factors was partially reversed by NFκB inhibition.
- These findings indicate CaSR activation elevates cytokine and chemokine production, partly mediated by NFκB.
Conclusions:
- CaSR plays a significant role in obesity-induced adipose tissue dysfunction.
- CaSR activation contributes to adipose tissue inflammation through NFκB signaling.
- Targeting CaSR presents a potential therapeutic strategy for managing obesity-related complications.
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