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Related Experiment Video

Updated: May 23, 2026

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices
10:35

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices

Published on: March 15, 2018

Two-photon calcium imaging in the intact brain.

Marco Dal Maschio1, Riccardo Beltramo, Angela Michela De Stasi

  • 1Department of Neuroscience and Brain Technologies, Italian Institute of Technology, Via Morego 30, 16163 Genoa, Italy. marco.dalmaschio@iit.it

Advances in Experimental Medicine and Biology
|March 29, 2012
PubMed
Summary

Calcium imaging measures cellular excitability in brain cells. This technique revolutionizes understanding of neural circuits and brain function in awake animals.

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Area of Science:

  • Neuroscience
  • Cellular Biology
  • Biophysics

Background:

  • Calcium ions are critical second messengers in brain cell pathophysiology.
  • Cellular excitability in neurons and glial cells is fundamental to nervous system function.

Purpose of the Study:

  • To detail methods for measuring calcium fluctuations as indicators of cellular excitability.
  • To review how in vivo calcium imaging advances the understanding of brain circuits.
  • To discuss future advancements in optical investigation of brain function.

Main Methods:

  • In vivo two-photon fluorescence calcium imaging techniques are described.
  • Focus on measuring calcium dynamics in intact central nervous system.
  • Application in awake, behaving animal models.

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In Vivo 2-Photon Calcium Imaging in Layer 2/3 of Mice
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In Vivo 2-Photon Calcium Imaging in Layer 2/3 of Mice

Published on: March 13, 2008

Long-Term Imaging of Identified Neural Populations using Microprisms in Freely Moving and Head-Fixed Animals
06:25

Long-Term Imaging of Identified Neural Populations using Microprisms in Freely Moving and Head-Fixed Animals

Published on: January 19, 2024

Related Experiment Videos

Last Updated: May 23, 2026

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices
10:35

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices

Published on: March 15, 2018

In Vivo 2-Photon Calcium Imaging in Layer 2/3 of Mice
08:10

In Vivo 2-Photon Calcium Imaging in Layer 2/3 of Mice

Published on: March 13, 2008

Long-Term Imaging of Identified Neural Populations using Microprisms in Freely Moving and Head-Fixed Animals
06:25

Long-Term Imaging of Identified Neural Populations using Microprisms in Freely Moving and Head-Fixed Animals

Published on: January 19, 2024

Main Results:

  • Calcium imaging provides a powerful reporter of neuronal and glial excitability.
  • This technique is revolutionizing the study of brain circuits at the cellular level.
  • Recent advancements enable optical investigation of brain function in dynamic states.

Conclusions:

  • In vivo calcium imaging is a key technology for studying brain function.
  • The technique offers new insights into neural circuit dynamics.
  • Future developments will further enhance optical methods for brain research.