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Updated: May 23, 2026

Rapid Point-of-Care Assay of Enoxaparin Anticoagulant Efficacy in Whole Blood
Published on: October 12, 2012
[Experimental studies on anticoagulant activity of different extract of leech in vitro]
Yanling Li1, Rongqing Huang, Xiaodong Sun
1Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850.
Objective:
To study the anticoagulant activity of different extracts of Leech.
Methods:
Prothrombin time (PT), thrombin time (TT) and activated partial thromboplastin time (APTT) were determined by coagulometer for blood samples collected from abdominal vein of Wistar rat.
Results:
PT, TT, APTT were prolonged remarkably by ethyl acetate part of Leech. PT was prolonged remarkably by hexane part and aqueous solution part of Leech. APTT was prolonged remarkably by n-Butylalcohol part of Leech.
Conclusion:
The different extracts of Leech have obvious anticoagulant effects. There are new anticoagulant components different from hirudin in Leech.
