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Related Concept Videos

Aryldiazonium Salts to Azo Dyes: Diazo Coupling01:11

Aryldiazonium Salts to Azo Dyes: Diazo Coupling

The reaction of weakly electrophilic aryldiazonium (also called arenediazonium) salts with highly activated aromatic compounds leads to the formation of products with an —N=N— link, called an azo linkage. This reaction, presented in Figure 1, is known as diazo coupling and occurs without the loss of the nitrogen atoms of the aryldiazonium salt. Highly activated aromatic compounds such as phenols or arylamines favor the diazo coupling reaction. The coupling generally occurs at the para position.
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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Related Experiment Video

Updated: May 23, 2026

Characterizing Mammalian Zinc Transporters Using an In Vitro Zinc Transport Assay
07:55

Characterizing Mammalian Zinc Transporters Using an In Vitro Zinc Transport Assay

Published on: June 2, 2023

Coumarin-derived transformable fluorescent sensor for Zn2+.

Zhaochao Xu1, Xin Liu, Jie Pan

  • 1Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. zcxu@dicp.ac.cn

Chemical Communications (Cambridge, England)
|April 5, 2012
PubMed
Summary
This summary is machine-generated.

A new coumarin-derived fluorescent sensor, CTS, selectively detects Zn(2+) by transforming its chelation form. This sensor distinguishes Zn(2+) from other metal ions through a unique tautomeric shift.

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Last Updated: May 23, 2026

Characterizing Mammalian Zinc Transporters Using an In Vitro Zinc Transport Assay
07:55

Characterizing Mammalian Zinc Transporters Using an In Vitro Zinc Transport Assay

Published on: June 2, 2023

High-throughput Screening for Small-molecule Modulators of Inward Rectifier Potassium Channels
10:07

High-throughput Screening for Small-molecule Modulators of Inward Rectifier Potassium Channels

Published on: January 27, 2013

Area of Science:

  • Analytical Chemistry
  • Chemical Sensing
  • Fluorescence Spectroscopy

Background:

  • Zinc(II) ions play crucial roles in biological systems.
  • Selective detection of Zn(2+) is essential for biological and environmental monitoring.
  • Existing sensors often lack specificity or exhibit interference from other metal ions.

Purpose of the Study:

  • To develop a novel coumarin-derived fluorescent sensor for selective Zn(2+) detection.
  • To investigate the sensing mechanism and selectivity of the developed sensor.
  • To demonstrate the sensor's utility in distinguishing Zn(2+) from other metal ions.

Main Methods:

  • Synthesis of a coumarin-derived fluorescent sensor (CTS).
  • Spectroscopic analysis (fluorescence) to evaluate sensor performance.
  • Metal ion binding studies to assess selectivity against competing ions.

Main Results:

  • CTS exhibits high fluorescence response towards Zn(2+).
  • CTS demonstrates excellent binding selectivity for Zn(2+) over various competing metal ions.
  • The selectivity is attributed to a transformable chelation mechanism involving tautomeric shifts.

Conclusions:

  • The coumarin-derived sensor CTS offers a selective and sensitive method for Zn(2+) detection.
  • The unique transformable chelation mechanism provides a basis for highly specific metal ion sensing.
  • CTS holds potential for applications in biological and environmental analysis requiring Zn(2+) quantification.