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Related Concept Videos

Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
Affinity Chromatography01:03

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...

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Related Experiment Video

Updated: May 23, 2026

Affinity Purification of a 6X-His-Tagged Protein using a Fast Protein Liquid Chromatography System
07:19

Affinity Purification of a 6X-His-Tagged Protein using a Fast Protein Liquid Chromatography System

Published on: April 26, 2024

Antibody affinity purification using metallic nickel particles.

Jun Gao1, Zhijun Li, Thomas Russell

  • 1Key Lab of Colloid and Interface Chemistry, Ministry of Education, Institute of Theoretical Chemistry, School of Chemistry & Chemical Engineering, Shandong University, Jinan 250100, PR China.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|April 6, 2012
PubMed
Summary
This summary is machine-generated.

Novel nickel ferromagnetic particles coated with Protein A offer rapid and stable antibody purification. This efficient method minimizes protein leaching, enabling scalable applications in research and pre-clinical studies.

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Last Updated: May 23, 2026

Affinity Purification of a 6X-His-Tagged Protein using a Fast Protein Liquid Chromatography System
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Published on: April 26, 2024

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Quantification of Metal Leaching in Immobilized Metal Affinity Chromatography

Published on: January 17, 2020

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
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Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

Published on: April 1, 2017

Area of Science:

  • Biotechnology
  • Materials Science
  • Immunology

Background:

  • Functionalized magnetic particles are increasingly utilized for biomacromolecule purification and cell separation.
  • Protein A is a key ligand for antibody affinity purification.

Purpose of the Study:

  • To develop and evaluate novel solid nickel ferromagnetic particles coated with Protein A for antibody affinity purification.
  • To assess the efficiency, stability, and scalability of this purification method.

Main Methods:

  • Utilized novel solid nickel ferromagnetic particles coated with Protein A.
  • Investigated IgG purification from undiluted mouse serum.
  • Employed protein crosslinking to stabilize Protein A on particle surfaces.

Main Results:

  • Achieved IgG purification from undiluted mouse serum in 5 minutes.
  • Demonstrated minimized Protein A leaching through protein crosslinking.
  • Showcased a gentle, scalable, automatable, efficient, and economical separation procedure.

Conclusions:

  • Protein A-coated nickel particles provide a rapid and stable method for antibody purification.
  • Protein crosslinking enhances the durability of the purification system.
  • The developed method is adaptable for various biological sample preparation and chromatographic applications, suitable for lab and pre-clinical research.