Related Experiment Video
Updated: May 23, 2026

Controlled-release of Chlorine Dioxide in a Perforated Packaging System to Extend the Storage Life and Improve the Safety of Grape Tomatoes
Published on: April 7, 2017
Gaseous chlorine dioxide as an alternative for bedbug control.
Shawn G Gibbs1, John J Lowe, Philip W Smith
1Department of Environmental, Agricultural and Occupational Health, University of Nebraska Medical Center College of Public Health, Omaha, Nebraska 68198, USA. sgibbs@unmc.edu
Gaseous chlorine dioxide (ClO(2)) effectively eradicates bedbugs, including eggs, in laboratory settings. This pest control method shows promise for use in hospital rooms, achieving high bedbug mortality with no residuals.
Area of Science:
- Pest control
- Environmental science
- Public health
Background:
- Bedbug infestations are a growing concern, posing significant challenges for eradication.
- Current bedbug control methods are often labor-intensive and require multiple treatments.
Purpose of the Study:
- To assess the effectiveness of gaseous chlorine dioxide (ClO(2)) against common bedbug species (Cimex lectularius and Cimex hemipterus).
- To determine the penetration capabilities of ClO(2) in a simulated hospital room environment.
Main Methods:
- Bedbugs were exposed to varying concentrations of ClO(2) (362, 724, 1,086 ppm) in a controlled laboratory setting.
- Bedbug mortality rates were recorded immediately post-exposure and at later time points.
- Bacillus atropheus spores were used as a biological indicator to evaluate ClO(2) penetration in a hospital room setting.
Main Results:
- Concentrations of 724 and 1,086 ppm ClO(2) resulted in immediate 100% mortality of adult bedbugs.
- No viable bedbug eggs were observed after ClO(2) exposure.
- Lower concentrations (362 ppm) achieved 100% mortality within 6 to 18 hours, depending on exposure duration (ppm-hours).
- A significant reduction (up to 6-log) in B. atropheus spores demonstrated effective ClO(2) gas distribution within a hospital room.
Conclusions:
- Gaseous chlorine dioxide is a highly effective agent for bedbug extermination in both laboratory and simulated room environments.
- Achievable concentrations in a hospital room setting are sufficient for bedbug eradication.
- ClO(2) treatment leaves no harmful residuals, making it a potentially safe and efficient pest control solution.
Related Concept Videos
Chemical Agents for Microbial Control
Microbial Bioremediation of Pesticides
Methods of Sterilization II: Chemical Methods
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
Biological Methods for Microbial Control
Production of Biopesticides
Radical Substitution: Halogenation of Alkanes and Alkyl Substituents
In the initiation step of the reaction, the chlorine molecule undergoes homolytic cleavage in the presence of light or heat, forming two highly reactive chlorine radicals. Propagation occurs in two...

