Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 23, 2026

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

Mesofluidic devices for DNA-programmed combinatorial chemistry.

Rebecca M Weisinger1, Robert J Marinelli, S Jarrett Wrenn

  • 1Department of Chemistry, Stanford University, Stanford, California, United States of America.

Plos One
|April 6, 2012
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pain, Nausea, and Hospital Admission after Uterine Fibroid Embolization: A Comparison of 2 Protocols.

Journal of vascular and interventional radiology : JVIR·2022
Same author

Advances in Proximity Ligation <i>in situ</i> Hybridization (PLISH).

Bio-protocol·2021
Same author

Progenitor identification and SARS-CoV-2 infection in human distal lung organoids.

Nature·2020
Same author

Progenitor identification and SARS-CoV-2 infection in long-term human distal lung organoid cultures.

bioRxiv : the preprint server for biology·2020
Same author

Single-cell Wnt signaling niches maintain stemness of alveolar type 2 cells.

Science (New York, N.Y.)·2018
Same author

Automated cell-type classification in intact tissues by single-cell molecular profiling.

eLife·2018
Same journal

Analysis of strength degradation of coal and rock masses and stability of mined areas under long term immersion environment.

PloS one·2026
Same journal

Biogenic Silver-Selenium nanocomposite with anticancer activity and potent efficacy against vancomycin-resistant Staphylococcus aureus.

PloS one·2026
Same journal

Preparation and physicochemical characterization of a biodegradable chitosan/carboxymethyl cellulose hydrogel synthesized in NaOH/urea medium.

PloS one·2026
Same journal

Action-guilt, survivor-guilt, and depression in combat-related PTSD.

PloS one·2026
Same journal

Explainable machine learning for predicting activities of daily living at discharge in stroke patients: A retrospective study using SHAP interpretability.

PloS one·2026
Same journal

Deep learning based two-way feature depiction model for brain tumor detection.

PloS one·2026
See all related articles

We developed a DNA-programmed chemical synthesis method using microwell plates for efficient molecular discovery. This high-throughput approach enables robust chemical reactions, advancing in vitro evolution of small molecules.

Area of Science:

  • Biochemistry
  • Organic Chemistry
  • Molecular Biology
  • Chemical Synthesis

Background:

  • DNA-hybrid combinatorial chemistry is a powerful approach for molecular discovery.
  • Existing methods require enhancement for higher throughput and broader applicability.

Purpose of the Study:

  • To present a highly parallel DNA-programmed chemical library synthesis format.
  • To demonstrate compatibility with automation and diverse synthetic schemes.
  • To validate the robustness and specificity of the method for molecular evolution.

Main Methods:

  • Utilized a standard microwell plate footprint for high-throughput synthesis.
  • Integrated fluidic routing for precise DNA population management.
  • Employed combinatorial synthetic schemes with up to 384 building blocks per step.

More Related Videos

Bilayer Microfluidic Device for Combinatorial Plug Production
07:03

Bilayer Microfluidic Device for Combinatorial Plug Production

Published on: December 1, 2023

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
14:36

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Published on: August 26, 2009

Related Experiment Videos

Last Updated: May 23, 2026

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

Bilayer Microfluidic Device for Combinatorial Plug Production
07:03

Bilayer Microfluidic Device for Combinatorial Plug Production

Published on: December 1, 2023

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
14:36

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Published on: August 26, 2009

Main Results:

  • Achieved excellent specificity in fluidic routing of DNA populations.
  • Demonstrated robust chemical synthesis on DNA arrayed in 384-well plates.
  • Validated the method's suitability for high-fidelity translation and in vitro evolution.

Conclusions:

  • The developed format significantly enhances DNA-programmed chemical library synthesis.
  • This method supports diverse combinatorial schemes and is compatible with automation.
  • It provides a robust platform for efficient discovery and evolution of small molecules.