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Published on: April 24, 2016
Ischemic preconditioning reduces transplanted submandibular gland injury
Ning-Yan Yang1, Liang Shi, Yan Zhang
1Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing, People's Republic of China.
The Journal of Surgical Research
|April 7, 2012
Summary
Ischemic preconditioning (IPC) protects transplanted rabbit submandibular glands from injury. IPC reduces inflammation, oxidative stress, and apoptosis, preserving gland function after transplantation.
Area of Science:
- Regenerative Medicine
- Transplantation Biology
- Surgical Innovation
Background:
- Ischemic preconditioning (IPC) is known to mitigate ischemia/reperfusion (I/R) injury in various organs.
- The protective effects of IPC on transplanted submandibular glands have not been previously investigated.
- Understanding IPC's role is crucial for improving outcomes in salivary gland transplantation.
Purpose of the Study:
- To investigate the protective effects of IPC on transplanted submandibular glands in a rabbit model.
- To elucidate the underlying mechanisms by which IPC confers protection.
- To assess the impact of IPC on gland function, inflammation, oxidative stress, and apoptosis.
Main Methods:
- Ischemic preconditioning involved 10 minutes of lingual artery clamping followed by 10 minutes of reperfusion before gland transplantation.
- Rabbits were divided into control, transplantation, and IPC groups (n=6 each).
- Saliva secretion, oxidative stress markers (ROS, MDA, MPO, SOD), pro-inflammatory cytokines (TNF-α, IL-1β), and apoptosis-related proteins (Bax, Bcl-2) were measured post-reperfusion.
Main Results:
- IPC treatment preserved salivary secretion at 24 hours post-reperfusion compared to the non-IPC group.
- IPC significantly attenuated increases in pro-inflammatory cytokines, oxidative stress markers (MDA, MPO), and reactive oxygen species (ROS) while enhancing superoxide dismutase (SOD) activity.
- IPC reduced acinar cell apoptosis and modulated Bax and Bcl-2 protein expression, indicating an anti-apoptotic effect.
Conclusions:
- Ischemic preconditioning demonstrates a protective effect on transplanted submandibular glands in rabbits.
- IPC mitigates I/R injury by suppressing inflammatory responses and oxidative stress.
- IPC promotes an anti-apoptotic process, thereby preserving the secretory function of the transplanted gland.
