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Updated: May 23, 2026
Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix
Published on: October 20, 2021
[Humoral and cellular immune responses to HBV in the blood donors]
Yong-Lin Yang1, Zu-Hu Huang, Xu-Bing Cai
1Nanjing Red Cross Blood Center, Nanjing 210003, China.
Aim:
To study the magnitude and correlation of humoral and cellular immune responses to hepatitis surface antigen(HBsAg), preS1+S2 antigen, and core antigen(HBcAg) in the blood donors.
Methods:
Enzyme linked immunosorbent assay (ELISA) was employed to scan the humoral immune responses to HBsAg, preS1+S2 antigen, and HBcAg. Enzyme linked immunospot assay (ELISPOT) was used to check the antigen specific IFN-γ secreting cells stimulated by HBsAg, preS1+S2 antigen or HBcAg in peripheral blood mononuclear cell from blood donors.
Results:
The prevalence of humoral immune response to preS1+S2 antigen was as high as 85.7% (48/56), compared with the lower response to HBcAg (30.4%, 17/56). In the cellular immune response, there was no difference between preS1+S2 antigen and HBcAg, both of which were stronger than the response stimulated by HBsAg. PreS1+S2 antigen or HBsAg specific humoral immune and cellular immune responses were highly correlated, which could not be observed in the HBcAg specific humoral immune and cellular immune responses(P>0.05).
Conclusion:
Either humoral or cellular immune responses to HBsAg, preS1+S2 antigen and HBcAg could be detected in the blood donors. PreS1+S2 antigen immune responses, however, were most powerful, which may suggest that PreS1+S2 antigen may be a candidate for the further HBV vaccine.
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