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Quantitative 1H NMR. Development and potential of an analytical method: an update
Guido F Pauli1, Tanja Gödecke, Birgit U Jaki
1Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, Chicago, Illinois 60612, USA. gfp@uic.edu
Journal of Natural Products
|April 10, 2012
Summary
Quantitative (1)H NMR (qHNMR) is a precise analytical method for natural product analysis. This review highlights its advantages, including external calibration and simultaneous quantitation, supporting its value in research.
Area of Science:
- Analytical Chemistry
- Natural Product Chemistry
Background:
- Quantitative (1)H NMR (qHNMR) is an established technique for analyzing chemical compounds.
- A previous literature overview covered qHNMR methodology and applications up to mid-2004.
Purpose of the Study:
- To provide an updated review of quantitative (1)H NMR (qHNMR) methodology and its applications in natural product analysis.
- To summarize new developments and experiential evidence from mid-2004 to the end of 2011.
Main Methods:
- Literature review covering publications from mid-2004 to end-2011.
- Analysis of over 170 new references detailing qHNMR methodology and applications.
Main Results:
- qHNMR offers advantages such as accurate external calibration and no need for identical reference materials.
- The method demonstrates high precision and accuracy when properly validated.
- qHNMR enables simultaneous quantitation of multiple analytes.
Conclusions:
- The updated literature review confirms qHNMR as a valuable and unbiased analytical tool.
- New methodology and evidence support the expanded use of qHNMR in natural product research and beyond.
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