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A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions
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A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions

Published on: June 6, 2018

Two-hybrid Systems * Stage 1: Characterization of a Bait-LexA Fusion Protein

Joseph Sambrook, David W Russell

    CSH Protocols
    |April 10, 2012
    PubMed
    Abstract

    No abstract available in PubMed .

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    Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...

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