Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
Tissue Homogenization and Cell Lysis01:32

Tissue Homogenization and Cell Lysis

Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods.
Mechanical methods of tissue homogenization
These methods rely on applying external physical force to disrupt...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeting mutant p53: Evaluation of novel anti-p53<sup>R175H</sup> monoclonal antibodies as diagnostic tools.

Scientific reports·2025
Same author

Delivery to, and Reactivation of, the p53 Pathway in Cancer Cells Using a Grafted Cyclotide Conjugated with a Cell-Penetrating Peptide.

Journal of medicinal chemistry·2024
Same author

Fever-like temperature bursts promote competence development via an HtrA-dependent pathway in Streptococcus pneumoniae.

PLoS genetics·2023
Same author

Proteasome inhibitor bortezomib stabilizes and activates p53 in hematopoietic stem/progenitors and double-negative T cells in vivo.

Proceedings of the National Academy of Sciences of the United States of America·2023
Same author

The application of dual energy X-ray soil screening in forensic archaeology.

Science & justice : journal of the Forensic Science Society·2022
Same author

Correction to: Identification of pathways modulating vemurafenib resistance in melanoma cells via a genome-wide CRISPR/Cas9 screen.

G3 (Bethesda, Md.)·2022
Same journal

Identification of positive GATEWAY expression clones when both the pENTRY and pDEST vectors contain the same marker for bacterial selection.

CSH protocols·2012
Same journal

Imaging protein interactions by FRET microscopy: cell preparation for FRET analysis.

CSH protocols·2012
Same journal

Imaging protein interactions by FRET microscopy: labeling proteins with fluorescent dyes.

CSH protocols·2012
Same journal

Bradford assay.

CSH protocols·2012
Same journal

Detection of ubiquitylated proteins in mammalian cells.

CSH protocols·2012
Same journal

Imaging of organelle membrane systems and membrane traffic in living cells.

CSH protocols·2012
See all related articles

Related Experiment Video

Updated: May 23, 2026

Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry
14:58

Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry

Published on: November 12, 2012

Immunoprecipitation: lysing bacteria by sonication.

Ed Harlow, David Lane

    CSH Protocols
    |April 10, 2012
    PubMed
    Summary
    This summary is machine-generated.

    This protocol uses intense ultrasound to lyse bacterial cells, breaking cell walls and shearing DNA for sample preparation. This method yields a lysate ready for preclearing, with minor sample denaturation.

    More Related Videos

    Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase
    08:59

    Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase

    Published on: February 12, 2019

    Chromatin Immunoprecipitation from Human Embryonic Stem Cells
    10:36

    Chromatin Immunoprecipitation from Human Embryonic Stem Cells

    Published on: July 22, 2008

    Related Experiment Videos

    Last Updated: May 23, 2026

    Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry
    14:58

    Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry

    Published on: November 12, 2012

    Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase
    08:59

    Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase

    Published on: February 12, 2019

    Chromatin Immunoprecipitation from Human Embryonic Stem Cells
    10:36

    Chromatin Immunoprecipitation from Human Embryonic Stem Cells

    Published on: July 22, 2008

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Biotechnology

    Background:

    • Bacterial cell lysis is a critical first step in many molecular biology workflows.
    • Efficient cell lysis is required to release intracellular contents, including DNA and proteins.
    • Traditional lysis methods can be time-consuming or inefficient.

    Purpose of the Study:

    • To present a protocol for rapid bacterial cell lysis using ultrasound.
    • To optimize DNA shearing during the lysis process.
    • To prepare bacterial lysates for subsequent molecular analyses.

    Main Methods:

    • Bacterial cells are subjected to short, intense ultrasound treatments.
    • Ultrasound energy breaks bacterial cell walls.
    • DNA is sheared to a specific size range during lysis.

    Main Results:

    • Effective lysis of bacterial cells is achieved.
    • DNA is sheared to sizes that do not impact sample viscosity.
    • Minor denaturation of cellular components occurs.

    Conclusions:

    • Ultrasound-based lysis is an efficient method for bacterial cell disruption.
    • The protocol provides a rapid and effective means to prepare lysates for preclearing.
    • This method is suitable for applications requiring DNA isolation and analysis.