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Updated: May 23, 2026

Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins
Published on: October 4, 2017
Modification of lysyl side chains using citraconic anhydride
Abstract:
INTRODUCTIONReversible masking of ε-amino groups of lysine is a valuable procedure for limiting trypsin hydrolysis of proteins to arginine peptide bonds. Citraconic anhydride is a reversible blocking agent of lysyl side chains. A major advantage of citraconylation is the ease with which the blocking group can be removed under conditions that will not lead to protein denaturation.
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