Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

SDS-PAGE

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Blinatumomab bypasses CD28 blockade to sustain T-cell cytotoxicity and improve survival in a xenograft B-ALL model.

Journal for immunotherapy of cancer·2026

Same author

Post-Transplant Bendamustine as a Platform for Immune Modulation After Allogeneic Hematopoietic Cell Transplantation.

European journal of haematology·2026

Same author

TIGIT Blockade Potentiates the Anti-Leukemic Activity of Exercise-Mobilized Donor Lymphocytes and Expanded γδ T-Cells.

Cancers·2026

Same author

Physical activity for public health in the 21st century.

Nature medicine·2026

Same author

Chronic exercise training intensity, immune cells, and cancer outcomes: a scoping review.

JNCI cancer spectrum·2026

Same author

Exercise-mobilized lymphocytes enhance antibody-based immunotherapy in multiple myeloma through CD16<sup>+</sup> NK cell-mediated cytotoxicity.

Journal of translational medicine·2026

Same journal

Identification of positive GATEWAY expression clones when both the pENTRY and pDEST vectors contain the same marker for bacterial selection.

CSH protocols·2012

Same journal

Imaging protein interactions by FRET microscopy: cell preparation for FRET analysis.

CSH protocols·2012

Same journal

Imaging protein interactions by FRET microscopy: labeling proteins with fluorescent dyes.

CSH protocols·2012

Same journal

Bradford assay.

CSH protocols·2012

Same journal

Detection of ubiquitylated proteins in mammalian cells.

CSH protocols·2012

Same journal

Imaging of organelle membrane systems and membrane traffic in living cells.

CSH protocols·2012

See all related articles

Search research articles

Related Experiment Video

Updated: May 23, 2026

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

Published on: October 6, 2017

Concentrating acrylamide gel spots.

Richard J Simpson

|April 10, 2012

Summary

This summary is machine-generated.

This protocol details a method to concentrate protein bands from gels using re-electrophoresis. This technique enhances protein yield for crucial sequencing applications.

More Related Videos

A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel

A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel

Published on: April 20, 2018

Preparation of Complaint Matrices for Quantifying Cellular Contraction

Preparation of Complaint Matrices for Quantifying Cellular Contraction

Published on: December 14, 2010

Related Experiment Videos

Last Updated: May 23, 2026

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

Published on: October 6, 2017

A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel

A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel

Published on: April 20, 2018

Preparation of Complaint Matrices for Quantifying Cellular Contraction

Preparation of Complaint Matrices for Quantifying Cellular Contraction

Published on: December 14, 2010

Area of Science:

Biochemistry
Proteomics

Background:

Protein sequencing often requires substantial sample amounts.
Excising and concentrating protein bands from gels is necessary for sufficient material.
Current methods may not be optimal for yield or ease of use.

Purpose of the Study:

To describe a simple and effective method for concentrating excised gel bands or spots.
To facilitate protein pre-concentration for subsequent sequencing analyses.

Main Methods:

Excising stained protein bands or spots from 1D or 2D acrylamide gels.
Re-electrophoresis of gel pieces within a simple SDS-PAGE gel.
Utilizing a conventional glass Pasteur pipette for gel casting and electrophoresis.

Main Results:

The described method effectively concentrates protein material from excised gel pieces.
Concentrated proteins are suitable for downstream applications like electroblotting.
The protocol provides sufficient material for amino-terminal or internal sequence analysis.

Conclusions:

This re-electrophoresis technique offers a practical approach to protein concentration from gels.
It is a valuable method for researchers requiring high-yield protein samples for sequencing.
The protocol is adaptable for both 1D and 2D gel electrophoresis applications.