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Fluoride uptake by Streptococcus mutans 6715.

G M Whitford, G S Schuster, D H Pashley

    Infection and Immunity
    |December 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

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    This study investigated Streptococcus mutans

    Area of Science:

    • Microbiology
    • Biochemistry
    • Dental Research

    Background:

    • Streptococcus mutans is a key cariogenic bacterium.
    • Understanding fluoride interaction with S. mutans is crucial for dental caries prevention.
    • Previous studies have explored fluoride's effects, but short-term uptake kinetics require further elucidation.

    Purpose of the Study:

    • To investigate the short-term kinetics of fluoride uptake by Streptococcus mutans.
    • To determine the influence of pH and pH gradients on fluoride accumulation within the cells.
    • To elucidate the mechanism of fluoride uptake in S. mutans.

    Main Methods:

    • Utilized rapid filtration and centrifugation techniques to study fluoride uptake kinetics.
    • Incubated S. mutans cultures (20-22h) in fluoride solutions buffered at various pH levels (2.0, 4.0, 5.5, 8.2).

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  • Measured fluoride concentration in the medium over time and determined cell-to-medium pH gradients using 14C-labeled 5,5-dimethyl-2,4-oxazolidinedione.

    • Main Results:

    • Fluoride uptake was inversely related to pH, being rapid and extensive at lower pH values.
    • Fluoride release from cells was observed as medium fluoride concentrations increased.
    • Fluoride uptake was significantly dependent on the magnitude of the pH gradient across the cell membrane (P < 0.001).
    • Glucose, cyanide, and iodoacetate did not affect fluoride uptake; preincubation in fluoride-free buffers reduced uptake.

    Conclusions:

    • Fluoride uptake by Streptococcus mutans is highly pH-dependent.
    • A significant pH gradient across the cell membrane drives fluoride accumulation.
    • The proposed mechanism involves the diffusion of hydrogen fluoride (HF) and subsequent intracellular trapping of ionic fluoride (F-).