[Research on serum prostate-specific antigen measurement by characteristic spectral absorption method]

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Medical Diagnostics

Background:

• Prostate-specific antigen (PSA) is a key biomarker for prostate cancer detection and monitoring.
• Accurate and accessible PSA measurement methods are crucial for effective clinical management.
• Existing methods may have limitations in terms of cost, speed, or complexity.

Purpose of the Study:

• To develop and validate a novel method for quantifying serum PSA levels.
• To utilize immunochromatography and spectral absorption for PSA measurement.
• To establish the feasibility and reliability of the proposed diagnostic approach.

Main Methods:

• Employing immunochromatography with colloidal gold-marked test-strips for PSA sample capture.
• Utilizing a spectral absorption method with specific LED irradiation (520-535 nm) and photodiode detection (520-540 nm).
• Calculating strip absorbency and determining the linear relationship with PSA concentration via linear fitting.

Main Results:

• A linear correlation was observed between serum PSA levels and test-strip absorbency within the 5 to 50 ng/mL range.
• Experiments using 21 different PSA standard solutions yielded a correlation coefficient of 0.910.
• The developed system demonstrated feasibility for serum PSA level measurement.

Conclusions:

• The proposed method combining immunochromatography and spectral absorption is a feasible approach for serum PSA measurement.
• This technique offers a potentially reliable and quantifiable method for assessing PSA levels.
• Further validation and clinical studies may establish its utility in prostate health monitoring.