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A novel nitrite biosensor based on gold dendrites with egg white as template.
Yaping He1, Dawei Zhang, Sheying Dong
1Institute of Analytical Science/Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, Xi'an, Shaanxi, P. R. China.
Summary
A novel nitrite biosensor was developed using gold dendrites templated by egg white and a myoglobin-L-cysteamine hybrid. This biosensor demonstrates sensitive detection of nitrite, offering potential for enzymatic biosensor design.
Area of Science:
- Electrochemistry
- Biosensor Technology
- Nanomaterials
Background:
- Egg white serves as an effective soft template for synthesizing gold dendrites (AuD).
- Myoglobin (Mb) is a key enzyme for electrochemical applications.
- L-cysteamine (Cys) facilitates the assembly of biological molecules onto nanomaterials.
Purpose of the Study:
- To fabricate a novel nitrite (NO(2)(-)) biosensor using a myoglobin-L-cysteamine-gold dendrite (Mb-Cys-AuD) bio-hybrid.
- To characterize the electrochemical properties and performance of the developed biosensor.
- To explore the potential of this bio-hybrid matrix for enzymatic biosensor design.
Main Methods:
- Synthesis of gold dendrites using egg white as a soft template.
- Assembly of a myoglobin-L-cysteamine-gold dendrite (Mb-Cys-AuD) bio-hybrid.
- Electrochemical characterization using cyclic voltammetry and electrochemical impedance spectroscopy.
- Fourier transform infrared spectra and UV-visible spectra to confirm Mb structure retention.
Main Results:
- The Mb-Cys-AuD hybrid retained the original structure of myoglobin.
- The biosensor exhibited well-defined redox peaks and a transfer rate constant of 1.49 s(-1).
- The biosensor showed sensitive electrochemical catalytic response for nitrite reduction with a linear range of 0.5–400 µM and a detection limit of 0.3 µM.
Conclusions:
- The Mb-Cys-AuD bio-hybrid is a promising matrix for constructing enzymatic biosensors.
- The developed nitrite biosensor demonstrates high sensitivity and stability.
- This approach offers new possibilities for designing biosensors with potential applications in various fields.
