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Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods
Published on: July 17, 2019
Association between STAT1 activity and BRAF mutations in papillary thyroid carcinomas.
Ji Hye Yim1, Eun Sook Kim, Hyun-Jung Choi
1Department of Internal Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.
Journal of Surgical Oncology
|April 20, 2012
Summary
Signal transducer and activator of transcription-1 (STAT1) activity is reduced in papillary thyroid carcinomas (PTCs). Lower STAT1 activity correlates with larger tumor size and BRAF mutations, suggesting its potential as a PTC biomarker.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Signal transducer and activator of transcription-1 (STAT1) is a tumor suppressor crucial in tumorigenesis.
- STAT1 regulates tumor cells and immune system functions.
- This study investigates STAT1 activity in human papillary thyroid carcinomas (PTCs).
Purpose of the Study:
- To evaluate STAT1 activity in human papillary thyroid carcinomas (PTCs).
- To determine the association between STAT1 activity and clinicopathologic factors in PTCs.
Main Methods:
- STAT1 activity was quantified in nuclear extracts from 35 PTC tumor tissues.
- An ELISA-based kit was employed for STAT1 activity measurement.
- Clinicopathologic factors were analyzed in relation to STAT1 activity.
Main Results:
- STAT1 activity was significantly lower in PTC tissues compared to normal thyroid tissues (P < 0.01).
- Lower STAT1 activity was observed in tumors ≥ 2 cm and inversely correlated with tumor size (rho = -0.34, P = 0.048).
- Tumors with BRAF mutations exhibited reduced STAT1 activity compared to wild-type tumors (P = 0.048).
Conclusions:
- STAT1 activity is suppressed in PTCs, indicated by decreased DNA-binding activities.
- Tumor size (≥ 2 cm) and the T1799A BRAF mutation are clinicopathologic factors linked to diminished STAT1 activity.
- STAT1 activity may serve as a potential biomarker for predicting PTC progression.
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