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[Study of the susceptibility of mice to Sendai strain Tianjin]
1Department of Microbiology, Tianjin Medical University, Tianjin 300070, China. zhaoguijin106@163.com
Abstract:
To explore the infectivity characteristics and susceptibility of Sendai strain Tianjin in 129Sv, DBA/2, Kunming and BALB/c mice and determine the optimal small rodent animal model for strain Tianjin research, the Sendai strain Tianjin was propagated for 72h in 9-11 day-old chicken embryos, the allantoic fluids were then harvested and the virus titer (1:1280) was detected by hemagglutination assay. Four different kinds of mice were intranasally inoculated with 5 microl and the diluted 30 microl virus solution. The weight loss of mice was monitored for 12 consecutive days and the survival rate was observed. Kunming and BALB/c mice were sacrificed on the first day prior to infection and on the fourth and seventh days post infection of the diluted 30 microl Sendai strain Tianjin. Their left lobes of lung were fixed with 4% formalin for histopathologic examination. The maximum percentage of average weight loss of 129Sv, DBA/2 were 13.0%, 4.7% with 100% survival rate when 129Sv, DBA/2, Kunming and BALB/c were inoculated with 5 microl virus solution, while the mice were inoculated with diluted 30 microl virus solution, the maximum percentage of average weight loss reached 21.7%, 30.3%, 16.7% and 9.7% with the survival rate of 20%, 0%, 80% and 100%. Lung infections of mice Kunming on the fourth and seventh day post infection were more severe than that of BALB/c, showing a large number of inflammatory cell exudation and thickening of the submucosa. It suggested that DBA/2 was the most susceptible to the infection of strain Tianjin. The mice susceptibility ranked as DBA/2>129Sv>Kunming>BALB/c. Mice DBA/2 and 129Sv could be used as the optimal small rodent animal models in the research of pathogenicity and vaccine of Sendai strain Tianjin.
Insights
DBA/2 mice are most susceptible to Sendai strain Tianjin infection, making them and 129Sv mice ideal models for studying this virus. This research helps identify optimal small rodent models for future Sendai virus studies.
Area of Science:
- Virology
- Immunology
- Animal Models
Background:
- Sendai virus is a significant respiratory pathogen.
- Understanding strain-specific infectivity is crucial for disease research.
- Identifying optimal animal models enhances experimental reproducibility.
Purpose of the Study:
- To evaluate the susceptibility of four mouse strains (129Sv, DBA/2, Kunming, BALB/c) to Sendai strain Tianjin.
- To determine the optimal small rodent animal model for research on Sendai strain Tianjin.
- To characterize the infectivity and pathogenicity of Sendai strain Tianjin.
Main Methods:
- Sendai strain Tianjin propagated in chicken embryos; virus titer determined by hemagglutination assay.
- Intranasal inoculation of mice with varying doses of the virus.
- Monitoring of weight loss, survival rates, and histopathological examination of lung tissues.
Main Results:
- DBA/2 mice exhibited the highest susceptibility to Sendai strain Tianjin, followed by 129Sv, Kunming, and BALB/c mice.
- Infection severity and mortality varied significantly across mouse strains and virus dosages.
- Histopathology revealed more severe lung inflammation in Kunming mice compared to BALB/c mice.
Conclusions:
- DBA/2 and 129Sv mice are identified as optimal small rodent models for studying Sendai strain Tianjin pathogenicity and vaccine development.
- The susceptibility ranking of the tested mouse strains is DBA/2 > 129Sv > Kunming > BALB/c.
- This study provides critical data for selecting appropriate animal models in Sendai virus research.
