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Updated: May 22, 2026

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
Simultaneous heterotrophic nitrification and aerobic denitrification by bacterium Rhodococcus sp. CPZ24
Peizhen Chen1, Ji Li, Qing X Li
1College of Resources and Environmental Sciences, China Agricultural University, Beijing 100193, China.
Abstract:
Rhodococcus sp. CPZ24 was isolated from swine wastewater and identified. Batch (0.25 L flask) experiments of nitrogen removal under aerobic growth conditions showed complete removal of 50 mg L(-1) ammonium nitrogen within 20 h, while nitrate nitrogen removal reached 67%. A bioreactor (50 L) was used to further assess the heterotrophic nitrification and aerobic denitrification abilities of Rhodococcus sp. CPZ24. The results showed that 85% of the ammonium nitrogen (100 mg L(-1)) was transformed to nitrification products (NO(3)(-)-N and NO(2)(-)-N) (13%), intracellular nitrogen (24%), and gaseous denitrification products (48%) within 25 h. The ammonium nitrogen removal rate was 3.4 mg L(-1)h(-1). The results indicate that the strain CPZ24 carries out simultaneous nitrification and denitrification, demonstrating a potential use of the strain for wastewater treatment.
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