Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
X-ray Imaging01:24

X-ray Imaging

German physicist Wilhelm Röntgen (1845–1923) was experimenting with electrical current when he discovered that a mysterious and invisible "ray" would pass through his flesh but leave an outline of his bones on a screen coated with a metal compound. In 1895, Röntgen made the first durable record of the internal parts of a living human: an "X-ray" image (as it came to be called) of his wife’s hand. Scientists worldwide quickly began their own experiments with X-rays, and by 1900, X-ray was widely...
X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal crystal...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optical control over topological Chern number in moiré materials.

Nature·2026
Same author

Building living systematic reviews and reporting standards for comparative microscopic analysis of white diseases in hard corals.

Ecology and evolution·2024
Same author

A retrospective analysis comparing set up errors from standard versus customised headrests for head and neck radiotherapy.

Radiography (London, England : 1995)·2022
Same author

Partner bereavement when parenting dependent children: What factors influence adjustment?

Death studies·2022
Same author

Anatomic Neuroimaging Characteristics of Posterior Fossa Type A Ependymoma Subgroups.

AJNR. American journal of neuroradiology·2021
Same author

Reflection on the proposed changes to dose quantities-an industrial perspective.

Journal of radiological protection : official journal of the Society for Radiological Protection·2021

Related Experiment Video

Updated: May 22, 2026

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
08:51

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Published on: May 27, 2008

Real space soft x-ray imaging at 10 nm spatial resolution.

W Chao1, P Fischer, T Tyliszczak

  • 1Center for X-ray Optics, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA. wlchao@lbl.gov

Optics Express
|April 27, 2012
PubMed
Summary
This summary is machine-generated.

Researchers achieved 10 nm spatial resolution in soft x-ray microscopy using advanced Fresnel zone plates. This breakthrough enables nanoscale studies with unprecedented detail.

More Related Videos

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography
07:01

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

Published on: October 24, 2019

Spectral Reflectometric Microscopy on Myelinated Axons In Situ
09:13

Spectral Reflectometric Microscopy on Myelinated Axons In Situ

Published on: July 2, 2018

Related Experiment Videos

Last Updated: May 22, 2026

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
08:51

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Published on: May 27, 2008

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography
07:01

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

Published on: October 24, 2019

Spectral Reflectometric Microscopy on Myelinated Axons In Situ
09:13

Spectral Reflectometric Microscopy on Myelinated Axons In Situ

Published on: July 2, 2018

Area of Science:

  • Physics
  • Materials Science
  • Nanotechnology

Background:

  • Soft x-ray microscopy is crucial for nanoscale imaging.
  • Achieving high spatial resolution is essential for detailed analysis.

Purpose of the Study:

  • To demonstrate 10 nm spatial resolution in soft x-ray microscopy.
  • To validate the use of robust nanofabrication processes for advanced microscopy.

Main Methods:

  • Fabrication of Fresnel zone plates using robust nanofabrication processes.
  • Utilizing both conventional full-field and scanning soft x-ray microscopes.

Main Results:

  • Successfully achieved 10 nm spatial resolution.
  • Demonstrated the effectiveness of the developed Fresnel zone plates.

Conclusions:

  • The achieved resolution marks a significant advancement in soft x-ray microscopy.
  • This development paves the way for true nanoscale studies.