Related Experiment Video
Updated: May 22, 2026
09:33
Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro
Published on: June 2, 2018
Making β cells from adult tissues
1Department of Human Molecular Genetics and Biochemistry, Sackler School of Medicine, Tel Aviv University, Ramat Aviv, 69978 Tel Aviv, Israel. sefrat@post.tau.ac.il
Trends in Endocrinology and Metabolism: TEM
|April 28, 2012
Summary
Exploring alternative diabetes therapies, this review focuses on using adult human cells for β-cell replacement. Strategies include expanding islet cells and reprogramming other cells, aiming for clinical application.
Area of Science:
- Endocrinology
- Stem Cell Biology
- Regenerative Medicine
Background:
- Type 1 diabetes treatment seeks effective beta-cell replacement strategies.
- Human pluripotent stem cells face challenges in generating functional insulin-producing cells.
- Adult human tissues offer an alternative source for beta-cell regeneration.
Purpose of the Study:
- To review progress in using adult human cells for diabetes therapy.
- To evaluate ex vivo expansion of human islet beta-cells.
- To examine nuclear reprogramming for converting non-beta cells into insulin-producing cells.
Main Methods:
- Review of current literature on beta-cell replacement strategies.
- Analysis of ex vivo expansion techniques for human islet beta-cells.
- Assessment of nuclear reprogramming methods for cell conversion.
Main Results:
- Significant advancements in deriving insulin-producing cells from adult human tissues.
- Progress in ex vivo expansion of human islet beta-cells.
- Development of nuclear reprogramming techniques for cell conversion.
Conclusions:
- Adult human cells present a promising alternative for beta-cell replacement in diabetes therapy.
- Further research is needed to overcome obstacles for clinical application.
- Both beta-cell expansion and cell reprogramming strategies show therapeutic potential.
More Related Videos
Related Concept Videos
01:25Insulin: Biosynthesis, Chemistry, and Preparation
The endoplasmic reticulum (ER) of pancreatic β-cells synthesizes preproinsulin, which consists of a signal peptide, A and B chains, and a C-peptide. Preproinsulin is then cleaved and folded into proinsulin, which translocates to the Golgi apparatus for sorting and packaging into secretory granules. In these granules, enzymatic clipping generates insulin and C-peptide.
Damage or functional impairment of β-cells inhibits insulin production, leading to diabetes. Diabetes treatment primarily uses...
Damage or functional impairment of β-cells inhibits insulin production, leading to diabetes. Diabetes treatment primarily uses...
01:23Tissue Renewal without Stem Cells
After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
However, failure of such a system...
However, failure of such a system...
01:22iPS Cell Differentiation
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.