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Updated: May 22, 2026
Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time
Published on: August 26, 2009
Detection of DNA based on fluorescence resonance energy transfer of polyelectrolyte-protected CdTe quantum dots as
Ting Qiu1, Bin Zhang, Zi-yang Hu
1College of Pharmaceutical Science, Soochow University, Suzhou, PR China.
Abstract:
The approach for DNA detection was established by using a fluorescence resonance energy transfer (FRET) system, in which the energy donor was poly-diallyldimethylammonium chloride-protected quantum dots and the energy receptor was ethidium bromide (EB) inserting into the double stranded DNA. The concentration of the probe DNA, EB and NaCl was optimized. Under the optimized conditions, the FRET system has a stable signal and good reproducibility. The linear range is 7.7-61.6 nM with the correlation coefficient of 0.998 and the limit of detection is 7.7 nM. This method is simple and sensitive, and makes the label-free DNA detection come true.
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