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Related Experiment Video

Updated: May 22, 2026

Intracranial Subarachnoidal Route of Infection for Investigating Roles of Streptococcus suis Biofilms in Meningitis in a Mouse Infection Model

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Published on: July 1, 2018

Lysozyme resistance in Streptococcus suis is highly variable and multifactorial.

Paul J Wichgers Schreur¹, Christian van Weeghel, Johanna M J Rebel

¹Central Veterinary Institute, Wageningen UR, Lelystad, The Netherlands.

|May 5, 2012

Summary

Streptococcus suis exhibits variable lysozyme resistance due to mechanisms like peptidoglycan modification, autolysin activity, and capsule production. Understanding these bacterial evasion strategies is crucial for controlling infections.

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Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Published on: March 3, 2023

Area of Science:

Microbiology
Immunology
Bacterial Pathogenesis

Background:

Streptococcus suis is a significant pathogen in swine and can infect humans.
The innate immune system, particularly lysozyme, is vital for combating S. suis infections.
Pathogens often develop mechanisms to resist lysozyme-mediated bacterial killing.

Purpose of the Study:

To compare lysozyme sensitivity across various S. suis isolates.
To investigate the molecular basis of lysozyme resistance in S. suis.

Main Methods:

Determined minimal inhibitory concentrations (MICs) of lysozyme for different S. suis strains.
Utilized gene inactivation (oatA, murMN operon) to assess their role in resistance.
Employed whole genome comparisons to identify single nucleotide polymorphisms (SNPs) linked to resistance.
Created isogenic mutants to validate the function of identified genes (autolysin, capsule sugar transferase).

Main Results:

Lysozyme MICs for S. suis isolates ranged from 0.3 to 10 mg/ml, indicating significant variability.
OatA-mediated peptidoglycan modification partially contributes to lysozyme resistance.
Inactivation of murMN operon showed additional peptidoglycan crosslinking is not involved in resistance.
SNPs correlated with resistance affected autolysin and capsule sugar transferase expression.
Mutant analysis confirmed autolysin activity and capsule structures are involved in lysozyme resistance.

Conclusions:

Lysozyme resistance in S. suis is highly variable across different isolates and serotypes.
Multiple mechanisms contribute to S. suis lysozyme resistance, including OatA-mediated peptidoglycan modification.
Autolysin activity and capsule production are also key factors in S. suis evading lysozyme-mediated killing.