Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Obtaining a representative blood sample in lactate tracer studies.

S L Lehman1, G A Brooks

  • 1Department of Physical Education, University of California, Berkeley.

Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Metabolisme
|September 1, 1990
PubMed
Summary

Venous tracer infusion with arterial sampling is superior for studying blood lactate kinetics. This method avoids inaccuracies associated with arterial infusion and venous sampling, offering a more reliable approach for research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Twelve weeks of endurance training increases FFA mobilization and reesterification in postmenopausal women.

Journal of applied physiology (Bethesda, Md. : 1985)·2010
Same author

Doublets and low-frequency fatigue in potentiated human muscle.

Acta physiologica Scandinavica·2005
Same author

Lactate shuttles in nature.

Biochemical Society transactions·2002
Same author

Lactate doesn't necessarily cause fatigue: why are we surprised?

The Journal of physiology·2001
Same author

Recovery of (13)CO2 during rest and exercise after [1-(13)C]acetate, [2-(13)C]acetate, and NaH(13)CO3 infusions.

American journal of physiology. Endocrinology and metabolism·2001
Same author

The effects of endurance training and exhaustive exercise on mitochondrial enzymes in tissues of the rat (Rattus norvegicus).

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology·2001

Area of Science:

  • Physiology
  • Biochemistry
  • Tracer Kinetics

Background:

  • Accurate measurement of blood lactate kinetics is crucial for understanding metabolic processes.
  • Existing methods using arterial infusion or venous sampling present significant limitations.
  • Arterial infusion can lead to biased tracer input due to streaming and uneven blood flow.
  • Venous sampling may not reflect true mixed venous blood, excluding cardiac and pulmonary contributions.

Purpose of the Study:

  • To present the advantages of venous tracer infusion with arterial sampling [(v-a) mode] over arterial infusion with venous sampling [(a-v) mode] for blood lactate kinetics studies.
  • To highlight the limitations of current methodologies in accurately assessing lactate turnover.

Main Methods:

  • Comparison of venous-arterial [(v-a)] and arterial-venous [(a-v)] tracer infusion modes.

Related Experiment Videos

  • Analysis of tracer input and sampling site representativeness for lactate kinetics.
  • Consideration of lactate metabolism in cardiac and pulmonary circulations.

    • Main Results:

    • Arterial tracer infusion can be impractical for human studies and prone to biased input.
    • Venous sampling does not accurately represent mixed venous blood or cardiac/pulmonary lactate metabolism.
    • Calculated turnover rates from venous or arterial sampling often underestimate true tissue turnover.
    • The (v-a) mode offers a more accurate representation of blood lactate kinetics.

    Conclusions:

    • The venous tracer infusion with arterial sampling [(v-a) mode] is advantageous for studying blood lactate kinetics.
    • This method overcomes the limitations of arterial infusion and venous sampling, providing more reliable data.
    • Accurate assessment of lactate turnover requires careful consideration of sampling methods and tissue contributions.