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Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...

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Updated: May 22, 2026

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos

Published on: August 20, 2017

Optimized protocol for derivation of human embryonic stem cell lines.

María Vicenta Camarasa¹, Víctor Miguel Galvez, Daniel Roy Brison

¹Caubet-Cimera Fundation, Centre for Advanced Respiratory Medicine, Recinte Hospital Joan March, Ctra Sóller km 12, 07110 Bunyola, Illes Balears, Mallorca, Spain. telomerasi@yahoo.es

Stem Cell Reviews and Reports

|May 23, 2012

Summary

This study details human embryonic stem cell (hESC) derivation protocols, offering quality control tips for optimizing embryo culture and inner cell mass isolation. It covers various culture systems for advancing hESC applications in regenerative medicine.

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Derivation and Characterization of a Transgene-free Human Induced Pluripotent Stem Cell Line and Conversion into Defined Clinical-grade Conditions

Derivation and Characterization of a Transgene-free Human Induced Pluripotent Stem Cell Line and Conversion into Defined Clinical-grade Conditions

Published on: November 26, 2014

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Last Updated: May 22, 2026

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos

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Published on: August 20, 2017

Derivation of Human Embryonic Stem Cells by Immunosurgery

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Derivation and Characterization of a Transgene-free Human Induced Pluripotent Stem Cell Line and Conversion into Defined Clinical-grade Conditions

Derivation and Characterization of a Transgene-free Human Induced Pluripotent Stem Cell Line and Conversion into Defined Clinical-grade Conditions

Published on: November 26, 2014

Area of Science:

Stem Cell Biology
Regenerative Medicine
Developmental Biology

Background:

Human embryonic stem cells (hESCs) have garnered significant scientific interest for their therapeutic potential.
Previous research has demonstrated hESC applications in treating spinal cord injury and blindness.
Established hESC derivation protocols often involve animal-derived components or complex feeder layers.

Purpose of the Study:

To outline critical steps in the human embryonic stem cell (hESC) line derivation protocol.
To provide quality control measures for each stage of hESC derivation.
To discuss advancements in hESC culture systems, including xeno-free and feeder-free methods.

Main Methods:

Optimization of early embryo culture conditions.
Precise and timely isolation of the inner cell mass (ICM).
Establishment of specific culture conditions to promote pluripotent cell growth.

Main Results:

Identification of key procedural steps crucial for successful hESC derivation.
Development of quality control strategies applicable throughout the derivation process.
Demonstration of various novel culture techniques (xeno-free, feeder-free, microdrops, suspension culture).

Conclusions:

Successful hESC derivation hinges on meticulous optimization of embryo culture, ICM isolation, and culture conditions.
Implementing quality control at each step is essential for reliable hESC line generation.
Advancements in culture systems offer improved methods for deriving and maintaining hESCs for research and clinical applications.