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Charged nanomatrices as efficient platforms for modulating cell adhesion and shape.

Jangho Kim1, Deok-Ho Kim, Ki Taek Lim

  • 1Department of Biosystems & Biomaterials Science and Engineering, Seoul National University, Seoul, Republic of Korea.

Tissue Engineering. Part C, Methods
|May 25, 2012
PubMed
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Charged nanomatrices, made from poly(ɛ-caprolactone) (PCL) and polyethylenimine, effectively control cell behavior. Positively charged nanomatrices (pPCL) enhance NIH 3T3 fibroblast cell adhesion and alter cell shape compared to negatively charged ones (nPCL).

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Nanotechnology

Background:

  • Cell behavior is influenced by the physical and chemical properties of their microenvironment.
  • Nanomaterials offer tunable platforms for controlling cellular responses.
  • Electrostatic interactions play a crucial role in cell-material interfaces.

Purpose of the Study:

  • To design and fabricate charged nanomatrices using poly(ɛ-caprolactone) (PCL) and polyethylenimine.
  • To investigate the effect of charged nanomatrices on NIH 3T3 fibroblast cell adhesion, affinity, and shape.
  • To explore the influence of electrostatic forces and adsorbed proteins on cell behavior.

Main Methods:

  • Fabrication of negatively charged PCL nanomatrices (nPCL) and positively charged PCL nanomatrices (pPCL) via electrospraying.

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  • Culturing NIH 3T3 fibroblast cells on nPCL and pPCL substrates.
  • Applying fluid shear stress to assess cell affinity.
  • Analyzing cell adhesion, shape, and cross-sectional morphology.
  • Characterizing serum protein adsorption on nanomatrix surfaces.

    • Main Results:

    • Positively charged pPCL nanomatrices significantly promoted NIH 3T3 fibroblast cell adhesion compared to nPCL nanomatrices.
    • Cell affinity on pPCL nanomatrices further increased under fluid shear stress.
    • NIH 3T3 cells exhibited distinct shapes: spherical on pPCL and aligned/narrow on nPCL.
    • Charged nanomatrices influenced cross-sectional cell shape, with cells being flattened on pPCL and rounder on nPCL.
    • Different serum proteins adsorbed onto nPCL and pPCL surfaces.

    Conclusions:

    • Charged nanomatrices are effective platforms for modulating cell behaviors, including adhesion and shape.
    • Nanoscale structure, electrostatic forces, and adsorbed biomolecules collectively influence cell responses.
    • The findings provide insights into designing biomaterials for controlled cell interactions.