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Related Experiment Videos

Improved enzymatic method to measure processing effects and starch availability in sorghum grain.

Y Xiong1, S J Bartle, R L Preston

  • 1Texas Tech University, Lubbock 79409.

Journal of Animal Science
|November 1, 1990
PubMed
Summary

A new enzymatic method accurately measures starch availability in steam-flaked sorghum grain (SFSG). This method correlates glucose release with processing effects and bulk density, offering a simple, fast, and sensitive approach for analysis.

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Area of Science:

  • Agricultural Science
  • Biochemistry
  • Food Science

Background:

  • Starch availability in sorghum grain is crucial for animal nutrition and processing efficiency.
  • Existing methods for measuring starch availability may not fully capture the effects of processing techniques like steam-flaking.

Purpose of the Study:

  • To develop and validate a modified enzymatic method for determining starch availability in steam-flaked sorghum grain (SFSG).
  • To correlate glucose release with processing parameters and physical characteristics of SFSG.

Main Methods:

  • Experiments were conducted to optimize enzyme concentration, reagents, particle size, shaking frequency, and buffer pH.
  • Glucose release was measured over 48 hours from uncooked, cooked, and steam-flaked sorghum grain samples.

Related Experiment Videos

  • Steam-flaked sorghum grain (SFSG) samples with varying bulk densities were produced by adjusting roller mill tension.

    • Main Results:

    • The modified enzymatic method accurately quantified glucose release, reflecting different degrees of starch gelatinization.
    • Glucose release from SFSG showed a strong correlation (r² = 0.99) with starch gelatinization.
    • Enzymatically determined glucose release values were more closely related to SFSG bulk density than birefringence measurements.

    Conclusions:

    • The developed enzymatic method is simple, fast, and sensitive for measuring starch availability in SFSG.
    • This method effectively assesses processing effects and provides a reliable alternative to birefringence for evaluating SFSG quality.