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Updated: May 22, 2026

In vitro Measurements of Tracheal Constriction Using Mice
Published on: June 25, 2012
Murine T cell-derived contractile activity for bronchial smooth muscle cells
Akemi Abe1, Satoshi Koyama, Takayuki Ohtomo
1Clinical Research Center for Allergy and Rheumatology, National Hospital Organization, Sagamihara National Hospital, Sagamihara, Japan.
Background:
Adoptive transfer of helper T (Th) cells conferred a late asthmatic response upon antigen challenge. A possible production of a contractile activity for bronchial smooth muscle (BSM) by activated Th cells was examined.
Method:
Murine Th clones were stimulated with immobilized anti-CD3 antibody in the presence or absence of anti-CD28 antibody. Culture supernatants were dialyzed and then applied to the collagen gels containing cultured human BSM cells.
Results:
Culture supernatants of activated but not resting murine Th clones that conferred a late asthmatic response, induced the contraction of human BSM cell-containing collagen gels.
Conclusion:
Activated Th cells produce a contractile activity for BSM in vitro.
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