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Published on: December 4, 2018
NFAT1 and NFAT2 differentially regulate IL-17A expression in human T cells
Osamu Kaminuma1, Noriko Kitamura, Akio Mori
1Department of Allergy and Immunology, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan. kaminuma-os@igakuken.or.jp
International Archives of Allergy and Immunology
|May 26, 2012
Summary
Nuclear Factor of Activated T cells (NFAT) 1 and 2 enhance Interleukin-17A (IL-17A) production in human T cells. NFAT2 showed a more vigorous effect on IL-17A expression than NFAT1.
Area of Science:
- Immunology
- Molecular Biology
- Transcription Factors
Background:
- Nuclear Factor of Activated T cells (NFAT) are key regulators of T cell function.
- NFAT proteins are increasingly recognized for their role in inflammatory cytokine production, including IL-17A.
- The specific contributions of different NFAT family members to IL-17A synthesis remain unclear.
Purpose of the Study:
- To investigate the distinct roles of NFAT1 and NFAT2 in regulating IL-17A expression in human T cells.
- To elucidate the functional differences between NFAT1 and NFAT2 in the context of IL-17A production.
Main Methods:
- Human cord blood CD4+ T cells were genetically modified using a lentiviral system to express NFAT1 or NFAT2.
- Quantitative real-time RT-PCR was employed to measure IL-17A mRNA levels.
- Transient expression assays in Jurkat-Tag cells were performed to assess the immediate impact of NFAT1 and NFAT2 on IL-17A.
Main Results:
- Overexpression of NFAT1 and NFAT2 led to increased IL-17A mRNA expression in human CD4+ T cells.
- NFAT2 demonstrated a more potent augmentation of IL-17A expression compared to NFAT1.
- NFAT1 enhanced IL-17A expression in Jurkat-Tag cells, while NFAT2 had no significant effect in this model.
Conclusions:
- Both NFAT1 and NFAT2 can facilitate IL-17A expression in human T cells.
- Distinct molecular mechanisms likely underlie the differential effects of NFAT1 and NFAT2 on IL-17A production.
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