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Updated: May 22, 2026
Isolation of Cancer Stem Cells From Human Prostate Cancer Samples
Published on: March 14, 2014
MRI of prostate stem cell antigen expression in prostate tumors
Jing Ren1, Zhuoli Zhang, Fang Wang
1Department of Radiology, Fourth Military Medical University, Xian 710032, China.
Background:
The prostate stem cell antigen (PSCA) is broadly overexpressed on the surface of prostate cancer cells.
Materials & Methods:
Anti-human PSCA monoclonal antibody (mAb 7F5) was bound to Fe(3)O(4)/Au (GoldMag) nanoparticles to serve as a PSCA-specific molecular MRI probe (mAb 7F5@GoldMag) for in vivo detection of prostate cancer cells. First, the efficacy of the antibody immobilization for the binding was assessed. Next, PC-3 (human prostate cancer cell line with PSCA overexpression) tumor-bearing mice were injected with mAb 7F5@GoldMag for MRI measurements while using mouse anti-human IgG bound to the particles (IgG@GoldMag) to serve as a nonspecific control. MRI examinations were conducted before and after injection of these probes at 6, 12 and 24 h; T2-weighted signal intensity within the tumors was measured.
Results:
Targeted binding of the mAb 7F5@GoldMag probe to PC-3 tumors was verified with optical images and MRI; selective binding was not observed for the nonspecific IgG@GoldMag probe.
Conclusion:
MRI measurements suggest the promising efficacy of this new approach for targeted molecular imaging of prostate tumors.
