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Updated: May 21, 2026

Inducing Apical Periodontitis in Mice
Published on: August 6, 2019
Human β-defensin 2 and protease activated receptor-2 expression in patients with chronic periodontitis
Alexandre Lustosa Pereira1, Marinella Holzhausen, Gilson César Nobre Franco
1Department of Periodontology, University of Taubaté, SP, Brazil. alupe@terra.com.br
Objective:
Some previous studies have shown that gingipains, trypsin-like proteases produced by Porphyromonas gingivalis, up-regulate human β defensin-2 (HBD-2) mRNA expression through protease-activated receptor-2 (PAR(2)) in gingival epithelial cells. This study aimed at investigating salivary HBD-2 levels and crevicular PAR(2) mRNA expression in human chronic periodontitis and evaluating whether periodontal treatment affected this process.
Methods:
Salivary and gingival crevicular fluid (GCF) samples were collected from periodontally healthy (control) and chronic periodontitis patients at baseline and 50 days after non-surgical periodontal treatment. Salivary HBD-2, and GCF TNF-α levels were analysed by ELISA, and PAR(2) mRNA at the GCF was evaluated by RT-PCR.
Results:
P. gingivalis was significantly (p<0.05) more prevalent in patients with chronic periodontitis when compared to controls. This prevalence decreased after periodontal therapy (p<0.0001). The control group showed statistically significant lower levels of HBD-2, TNF-α, and PAR(2) expression when compared to the chronic periodontitis group. In addition, periodontal treatment significantly reduced PAR(2) expression and HBD-2 levels in chronic periodontitis patients (p<0.001).
Conclusions:
Our results suggest that salivary HBD-2 levels and PAR(2) mRNA expression from GCF are higher in subjects with chronic periodontitis than in healthy subjects, and that periodontal treatment decreases both HBD-2 levels and PAR(2) expression.
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