Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Development and validation of a prediction model for tuberculous peritoneal effusion.

Frontiers in medicine·2026

Same author

Mapping and engineering the human cell-cell interactome.

Nature biotechnology·2026

Same author

Persistent Benefits of Early Gestational Chenodeoxycholic Acid Supplementation on Late Pregnancy in Sows Via Sustained Modulation of the Gut-Metabolism Axis.

The Journal of nutrition·2026

Same author

Stress-hyperglycemia ratio and glycemic variability predict severity and mortality in sepsis-associated acute respiratory distress syndrome.

World journal of emergency medicine·2026

Same author

The temporal architecture of the seminiferous epithelial cycle revealed by spatial transcriptomics.

Cell·2026

Same author

Anti-fatigue mechanism of <i>Dendrobium officinale</i> pseudobulbs from different growth durations against chronic fatigue syndrome in mice and analysis of their active chemical components.

Frontiers in cell and developmental biology·2026

Search research articles

Related Experiment Video

Updated: May 21, 2026

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

Single-cell systems biology by super-resolution imaging and combinatorial labeling.

Eric Lubeck¹, Long Cai

¹Program in Biochemistry and Molecular Biophysics, California Institute of Technology, Pasadena, California, USA.

|June 5, 2012

Summary

Researchers developed a new method combining super-resolution microscopy and combinatorial labeling to simultaneously detect more molecules in single cells. This advance enables deeper insights into cellular regulatory networks and systems biology.

More Related Videos

Super-Resolution Live Cell Imaging of Subcellular Structures

Super-Resolution Live Cell Imaging of Subcellular Structures

Published on: January 13, 2021

Ground State Depletion Super-resolution Imaging in Mammalian Cells

Ground State Depletion Super-resolution Imaging in Mammalian Cells

Published on: November 5, 2017

Related Experiment Videos

Last Updated: May 21, 2026

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

Super-Resolution Live Cell Imaging of Subcellular Structures

Super-Resolution Live Cell Imaging of Subcellular Structures

Published on: January 13, 2021

Ground State Depletion Super-resolution Imaging in Mammalian Cells

Ground State Depletion Super-resolution Imaging in Mammalian Cells

Published on: November 5, 2017

Area of Science:

Cellular and Molecular Biology
Biophysics
Microscopy and Imaging

Background:

Fluorescence microscopy is vital for single-cell regulatory network analysis.
Current limitations exist in simultaneously measuring multiple molecular species due to spectral overlap of fluorophores.

Purpose of the Study:

To develop a general strategy for increasing multiplex detection capacity in single cells.
To demonstrate the combined use of optical super-resolution microscopy (SRM) and combinatorial labeling for enhanced molecular detection.

Main Methods:

Utilized fluorescence in situ hybridization (FISH) for labeling mRNAs with unique fluorophore combinations.
Employed optical super-resolution microscopy (SRM) to resolve fluorophore sequences and combinations.
Applied the method to measure mRNA levels of 32 genes simultaneously in single Saccharomyces cerevisiae cells.

Main Results:

Successfully demonstrated a strategy to significantly increase multiplex detection capacity in single cells.
Achieved simultaneous measurement of mRNA levels for 32 genes in individual yeast cells.
Resolved specific sequences and combinations of fluorophores using SRM.

Conclusions:

Combinatorial labeling coupled with SRM offers a powerful approach for high-capacity multiplex molecular detection in single cells.
This technique facilitates the integration of systems biology approaches at the single-cell level.
The method provides a foundation for more comprehensive analysis of cellular regulatory networks.